红托竹荪菌种快速分离培养基优化  

Optimization of Culture Medium for Rapid Strain Isolation of Red Volva Dictyophora(Dictyophora rubrovolvata)

作　　者：陈飞[1] 韩冰[1] 于广峰[1] 钟丽娟[1] 柴林山[1] 郭玲玲[1] CHEN Fei;HAN Bing;YU Guang-feng;ZHONG Li-juan;CHAI Lin-shan;GUO Ling-ling(Acad.of Microbiol.of Liaoning Prov.,Chaoyang 122000)

机构地区：[1]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《微生物学杂志》2023年第6期54-61,共8页Journal of Microbiology

基　　金：辽宁省“揭榜挂帅”科技攻关专项(2021JH/10400035-02);食药用菌学科建设项目(2022DD154625);辽宁省科学事业公益研究基金项目(2022JH4/10100111);辽宁省科技特派行动专项(2023020687-JH5/104)。

摘　　要：通过对红托竹荪快速分离培养基优化,提高红托竹荪菌种分离与评价效率。采用响应面分析法,以菌种生长速度为响应值拟合二次多元回归方程,确定培养基配方;测定优化培养基与PDA对照培养基菌丝生长速度和菌丝直径,以菌丝形态、锁状联合和菌落形态等指标评价优化培养基;测定优化培养基与PDA培养基培养菌丝在木屑培养基中菌丝生长速度,验证应用效果。通过试验,筛选出快速分离培养基配方为葡萄糖20.71 g/L、全麦粉8.36 g/L、玉米粉8.07 g/L、琼脂粉18.00 g/L、木屑水1.06 L。快速分离培养基与PDA培养基对比,培养的菌落直径平均增加66.25%,快速分离培养基菌丝日平均生长速度增加33.33%,木屑培养基菌丝日平均生长速度增加44.22%。由于优化培养基中含有淀粉、纤维素等有效成分,其刺激了菌种分泌淀粉酶、纤维素酶等,维持了胞外酶系的完整性。还可根据菌丝培养基过程形成的透明圈大小判定菌种胞外酶产生能力,达到快速评价菌种质量,保障菌种质量的目的。Efficiency of separation and evaluation of strain isolation medium of red volva dictyophora(Dictyophora rubrovolvata)was improved through optimization of strain rapid isolation medium of D.rubrovolvata,and the growth rate response value of the strain were adopted to fit the quadratic multivariate regression equation.Response surface analysis was used to determine the medium formula.The mycelial growth rate and mycelial diameter of the optimized medium and the control medium of PDA were measured,and observed the mycelial morphology,clamp connection,and colony morphology to evaluate the optimization of the medium.The growth rate of mycelia cultured by optimized medium and PDA medium in sawdust was measured to test and verify the application effect.The results showed that the optimized culture medium for rapid isolation was glucose 20.71 g/L,graham powder 8.36 g/L,corn flour 8.07 g/L,agar powder 18.00 g/L,and sawdust water 1.06 L.Compared optimized medium with PDA medium,the average mycelium diameter increased by 66.25%,the daily average growth rate on plate culture increased by 33.33%,and the daily average growth rate on sawdust day increased by 44.22%.The culture medium for strain rapid isolation contains starch,cellulose and other effective ingredient,which stimulates the strain to secrete amylase and cellulase etc,and maintain the integrity of extracellular enzyme system,and formed transparent circle during the culture process.The extracellular enzyme production capacity of the strains can be judged by the size of the transparent circle,and the quality of the strains can be rapidly evaluated to ensure the quality of the strains.

关 键 词：红托竹荪(Dictyophora rubrovolvata) 快速分离培养基 透明圈 生长速度 菌丝直径 

分 类 号：Q93-335[生物学—微生物学]