流行性乙型脑炎高免阳性血清的制备与应用  

Preparation and Application of Hyperimmune Serum of Epidemic Encephalitis B

作　　者：李翠[1] 王兆 王德丽 高月异 沈青春[1] 高金源[1] Li Cui;Wang Zhao;Wang Deli;Gao Yueyi;Shen Qingchun;Gao Jinyuan(China Institute of Veterinary Drug Control,Beijing 100081,China;Shandong First Medical University,,Jinan 250117,Shandong,China)

机构地区：[1]中国兽医药品监察所,北京100081 [2]山东第一医科大学,山东济南250117

出　　处：《中国动物检疫》2024年第1期102-106,共5页China Animal Health Inspection

基　　金：国家重点研发计划项目(2021YFD800500);兽药行业公益性重点专项(GY202014);山东省生猪产业技术体系项目(SDAIT-08-17)。

摘　　要：为满足乙型脑炎活疫苗的鉴别检验及外源病毒检验需求,以乙型脑炎活疫苗和灭活疫苗为免疫原,通过对SPF鸡进行反复免疫制备乙型脑炎特异性血清。当血清效价满足要求时,以心脏采血法采集血清,经分装、冻干后,开展纯净性检验、噬斑减少中和效价测定、特异性检验、剩余水分测定和均匀性检验,并对乙型脑炎活疫苗进行鉴别检验和外源病毒检验。结果显示:制备的乙型脑炎阳性血清无菌、支原体及外源病毒检验结果均为阴性,与口蹄疫病毒、猪圆环病毒、伪狂犬病病毒、猪瘟病毒、猪繁殖与呼吸综合征病毒、猪细小病毒和牛病毒性腹泻病毒等均无交叉反应,噬斑减少中和效价大于1:1 000,剩余水分<3%,且10个样品的效价测定结果组内、组间均无明显差异(P<0.05)。结果表明,制备的乙型脑炎阳性血清效价高,剩余水分少,纯净性、特异性、均匀性均良好,可满足乙型脑炎活疫苗的检验需求。In order to satisfy the needs of identification test and exogenous virus test for encephalitis B live vaccines,specific serum of encephalitis B was prepared by repeated immunization in SPF chickens taking live and inactivated vaccines as immunogens.When the serum titer was up to requirement,the serum was collected using the method for heart blood collection,after being packed and freeze-dried,then purity test,plaque reduction neutralization titer test,specificity test,residual water test and uniformity test were conducted,and identification test and exogenous virus test for encephalitis B live vaccines were carried.The results showed that the prepared positive serum was sterile,the test results of bacteria,Mycoplasma and exogenous viruses were all negative,and failed to crossly react with foot-and-mouth disease virus(FMDV),porcine circovirus(PCV),pseudorabies virus(PRV),classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),porcine parvovirus(PPV)and bovine viral diarrhea virus(BVDV).The titer of plaque reduction neutralization test was>1:1000,and the residual moisture was<3%,without obvious difference in the titer test results of 10 samples in intra and inter groups(P<0.05).In conclusion,the prepared serum was with high titer,less residual moisture,good purity,specificity and uniformity,and could meet the need of test for encephalitis B.

关 键 词：乙型脑炎 阳性血清 鉴别检验 外源病毒检验 

分 类 号：S852.6[农业科学—基础兽医学]