一种基于2-(2-羟基苯基)苯并噻唑近红外脂滴荧光探针的合成及细胞成像研究  

Synthesis and Cell Imaging of a 2-(2-Hydroxyphenyl)Benzothiazole-Based Near Infrared Lipid Droplets Fluorescence Probe

作　　者：于坤 王亚轩 沈舒婷 胡磊 王慧 YU Kun;WANG Yaxuan;SHEN Shuting;HU Lei;WANG Hui(Department of Pharmacy,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《杭州师范大学学报（自然科学版）》2024年第1期6-9,19,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家级大学创新创业训练计划项目(202110368056);皖南医学院大学生科研资助项目(WK2022XS11).

摘　　要：以2-(2-羟基苯基)苯并噻唑为母体,通过缩合反应,将其与二氰基异佛尔酮相连接,合成得到一种近红外荧光探针LD-YK,并对其结构进行了氢谱表征.通过紫外-可见吸收与荧光发射光谱研究了探针LD-YK的光物理性质,结果表明探针LD-YK在不同溶剂中的最大荧光发射波长均超过650 nm,属于近红外区域,且具有较大的斯托克斯位移.细胞实验结果表明探针LD-YK具有较低的细胞毒性,且可靶向于细胞内的脂滴部位.A near-infrared fluorescence probe LD-YK was synthesized by the condensation reaction using 2-(2-hydroxyphenyl)benzothiazole as the parent with dicyanoisophorone,which was characterized by 1H NMR spectroscopy.The photophysical properties of probe LD-YK were studied by UV-vis absorption spectrum and fluorescence emission spectrum.The results demonstrated that the maximum fluorescence emission wavelength of probe LD-YK in different solvents exceeded 650 nm,which belonged to the near infrared region and had a large Stokes shift.The cell experiment results showed that the probe LD-YK had low cytotoxicity and could target lipid droplets within the cell.

关 键 词：2-(2-羟基苯基)苯并噻唑 近红外 脂滴 异佛尔酮 荧光探针 

分 类 号：O625.6[理学—有机化学]