Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice  

作　　者：GAO Ningning YE Shuifeng ZHANG Yu ZHOU Liguo MA Xiaosong YU Hanxi LI Tianfei HAN Jing LIU Zaochang LUO Lijun 

机构地区：[1]College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]Shanghai Agrobiological Gene Center,Shanghai 201106,China [3]College of Life Sciences,Shangrao Normal University,Shangrao 334001,China [4]Institute for Agri-food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China

出　　处：《Rice science》2024年第1期62-76,共15页水稻科学（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2018YFE0106200);the Science and Technology Research Project of Jiangxi Provincial Department of Education,China(Grant No.K4100131);the Science and Technology Research Project of Shangrao,Jiangxi Province,China(Grant No.K4000019).

摘　　要：Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

关 键 词：antioxidant enzyme β-carotene ketolase drought resistance Nostoc flagelliforme osmotic stress RICE transcriptome analysis 

分 类 号：S511[农业科学—作物学]