OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice  

作　　者：DONG Xinli ZHOU Yang ZHANG Yaqi RONG Fuxi DU Jiahong HONG Zheyuan HU Peisong LÜYusong 

机构地区：[1]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [2]State Key Laboratory of Rice Biology and Breeding/China National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Rice science》2024年第1期77-86,I0018,I0019,共12页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.32101763);China National Postdoctoral Program for Innovative Talents(Grant No.BX2021266);China Postdoctoral Science Foundation(Grant No.2021M692853).

摘　　要：As the‘Green Revolution’gene,SD1(encoding GA20ox2),has been widely applied to improve yield in rice breeding.However,research on its transcriptional regulation is limited.Here,we identified a transcription factor OsbZIP01,which can suppress the expression of SD1 and regulate gibberellin(GA)biosynthesis in rice.Knockout mutants of OsbZIP01 exhibited increased plant height,while the overexpression lines showed a semi-dwarf phenotype and diminished germination rate.Furthermore,the semi-dwarf phenotype of OE-bZIP01,was caused by the reduced internode length,which was accompanied by a thin stem width.The predominant expression of OsbZIP01 was observed in leaves and sheaths.OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity.In addition,OsbZIP01 could directly bind to the promoter of the OsSD1 gene,and inhibit its transcription.The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA_(3).Meanwhile,the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type,indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway.Collectively,OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription,thereby affecting plant growth and development.

关 键 词：OsbZIP01 SD1 gibberellin biosynthesis dwarf and germination 

分 类 号：S511[农业科学—作物学]