Identification and Molecular Characterization of the Alkaloid Biosynthesis Gene Family in Dendrobium catenatum  

作　　者：Liping Yang Xin Wan Runyang Zhou Yingdan Yuan 

机构地区：[1]College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou,China [2]Jiangsu Academy of Forestry,Nanjing,China [3]Jiangsu Yangzhou Urban Forest Ecosystem National Observation and Research Station,Yangzhou,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第1期81-96,共16页国际实验植物学杂志（英文）

基　　金：the Forestry Science and Technology Innovation and promotion Project of Jiangsu Province‘Long-Term Research Base of Forest and Wetland Positioning Monitoring in Jiangsu Province’(Grant No.LYKJ[2020]21);Natural Science Foundation of Jiangsu Province,China(Grant No.BK20210800);the National Natural Science Foundation of China(Grant Nos.32001341 and 32202523);Jiangsu Agriculture Science and Technology Innovation Fund(Grant No.CX(21)3047).

摘　　要：As one of the main active components of Dendrobium catenatum, alkaloids have high medicinal value. The physicochemicalproperties, conserved domains and motifs, phylogenetic analysis, and cis-acting elements of the genefamily members in the alkaloid biosynthesis pathway of D. catenatum were analyzed by bioinformatics, and theexpression of the genes in different years and tissues was analyzed by qRT-PCR. There are 16 gene families,including 25 genes, in the D. catenatum alkaloid biosynthesis pathway. The analysis of conserved domains andmotifs showed that the types, quantities, and orders of domains and motifs were similar among members ofthe same family, but there were significant differences among families. Phylogenetic analysis indicated that thegene family members showed some evolutionary conservation. Cis-acting element analysis revealed that therewere a large number of light-responsive elements and MYB (v-myb avian myeloblastosis viral oncogene homolog)-related elements in these genes. qRT-PCR showed that expressions of gene family members involved in alkaloidsynthesis were different in different years and tissues of D. catenatum. This study provides a theoretical basisfor further exploration of the regulatory mechanisms of these genes in the alkaloid biosynthesis of D. catenatum.

关 键 词：Dendrobium catenatum gene family alkaloid biosynthesis 

分 类 号：Q94[生物学—植物学]