共生菌Rickettsia响应宿主烟粉虱成虫日龄的免疫基因表达调控作用  

Regulation of the symbiont Rickettsia on the expression of the immune genes in response to adult day-old ages of the host whitefly,Bemisia tabaci(Hemiptera:Aleyrodidae)

作　　者：赵冬晓 张志春[1] 牛洪涛[1] 郭慧芳[1] ZHAO Dong-Xiao;ZHANG Zhi-Chun;NIU Hong-Tao;GUO Hui-Fang(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《昆虫学报》2023年第12期1552-1559,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(32172412,32172410);江苏省农业科技自主创新资金项目(CX(23)1037)。

摘　　要：【目的】共生菌与宿主昆虫先天免疫有效协同是维持其与宿主互惠关系的重要生理基础。Rickettsia是烟粉虱Bemisia tabaci体内的优势共生菌,本研究旨在探索Rickettsia对烟粉虱不同日龄成虫免疫基因表达的调控作用。【方法】利用qRT-PCR检测和比较100%感染、45.83%感染和不感染共生菌Rickettsia的烟粉虱品系成虫中免疫关键因子溶菌酶基因BtLyz1和防御素基因Btdef的表达量;利用qRT-PCR检测100%感染Rickettsia的初羽化24 h内成虫、3-7日龄以及大于15日龄成虫中BtLyz1和Btdef及细胞凋亡基因BtCaspase和Rickettsia 16S rRNA基因的表达量;分析烟粉虱不同日龄成虫中Rickettsia 16S rRNA基因表达量与BtLyz1和Btdef表达量的相关性。【结果】与不感染共生菌Rickettsia的烟粉虱成虫相比,感染Rickettsia的烟粉虱成虫中BtLyz1和Btdef表达量显著提高。感染Rickettsia的大于15日龄烟粉虱成虫中BtCaspase表达量最高;在3-7日龄烟粉虱成虫中Rickettsia 16S rRNA基因、BtLyz 1和Btdef表达量显著高于初羽化24 h内和大于15日龄成虫中的。烟粉虱初羽化24 h内和3-7日龄成虫中Rickettsia 16S rRNA基因表达量与BtLyz 1和Btdef表达量呈显著正相关,而大于15日龄成虫中Rickettsia 16S rRNA基因表达量与BtLyz 1和Btdef表达量无相关性。【结论】共生菌Rickettsia能够上调宿主烟粉虱免疫相关基因表达量,但宿主机体开始衰老后该调控作用即明显下降。【Aim】The effective coordination between symbiont and host insect innate immunity is an important physiological basis for maintaining their reciprocal relationship.Rickettsia is a dominant symbiont in the whitefly,Bemisia tabaci.The aim of this study is to investigate the regulation of Rickettsia on the expression of the immune genes of B.tabaci adults at different day-old ages.【Methods】The expression levels of immune key factor lysozyme gene BtLyz1 and defensin gene Btdef in adult B.tabaci lines with 100%infection,45.83%infection and no infection by the symbiont Rickettsia were detected and compared,respectively,using qRT-PCR.The expression levels of BtLyz1 and Btdef,apoptosis gene BtCaspase and Rickettsia 16S rRNA gene in 100%Rickettsia-infected adults newly emerged within 24 h and adults at the 3-7-day-old and over 15-day-old were detected,respectively,using qRT-PCR.The correlations between the expression level of 16S rRNA gene of Rickettsia and the expression levels of BtLyz1 and Btdef in B.tabaci adults at different day-old ages were analyzed.【Results】The expression levels of BtLyz1 and Btdef in B.tabaci adults infected with the symbiont Rickettsia were significantly increased as compared to those in B.tabaci adults non-infected with Rickettsia.The highest expression level of BtCaspase was observed in B.tabaci adults at over 15-day-old infected with Rickettsia.The expression levels of Rickettsia 16S rRNA gene,BtLyz1 and Btdef in the 3-7-day-old adults of B.tabaci were significantly higher than those in adults newly emerged within 24 h and at over 15-day-old.The expression level of Rickettsia 16S rRNA gene was significantly positively correlated with the expression levels of BtLyz1 and Btdef in B.tabaci adults newly emerged within 24 h and at the 3-7-day-old,while there was no correlation between the expression level of Rickettsia 16S rRNA gene and the expression levels of BtLyz1 and Btdef in adults at over 15-day-old.【Conclusion】The symbiont Rickettsia can upregulate the expression levels of i

关 键 词：烟粉虱 共生菌 免疫基因 溶菌酶基因 防御素基因 

分 类 号：Q965.8[生物学—昆虫学]