致死中浓度阿维菌素对中国梨木虱短期内对阿维菌素的抗性发展的影响  

Effects of medium lethal concentration of avermectin on the development of short-term resistance of Psylla chinensis(Hemiptera:Psyllidae)to avermectin

作　　者：张蕊 张博晨 许双叶 吴梓情 马瑞燕[1] 李娅 张东霞 庾琴[1] ZHANG Rui;ZHANG Bo-Chen;XU Shuang-Ye;WU Zi-Qing;MA Rui-Yan;LI Ya;ZHANG Dong-Xia;YU Qin(College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,China;Shanxi Provincial Plant Protection and Quarantine Station,Taiyuan 030801,China)

机构地区：[1]山西农业大学植物保护学院,太原030031 [2]山西省植物保护检疫总站,太原030801

出　　处：《昆虫学报》2023年第12期1581-1589,共9页Acta Entomologica Sinica

基　　金：国家现代农业(梨)产业技术体系资助项目(CARS-28);山西农业大学博士基金(2021BQ56)。

摘　　要：【目的】明确致死中浓度(medium lethal concentration,LC_(50))的阿维菌素对中国梨木虱Psylla chinensis种群短期内对阿维菌素的抗性发展的影响。【方法】采用浸叶法测定阿维菌素处理24 h时对中国梨木虱田间种群(F)3龄若虫的LC_(50)值;室内条件下,以F种群为基础,建立中国梨木虱对阿维菌素相对敏感品系(SS)、F种群连续6代接触LC_(50)浓度的阿维菌素的田间抗性品系(FR)和6代未接触阿维菌素的田间对照品系(FS),测定中国梨木虱F种群和各品系3龄若虫对阿维菌素的抗性倍数,测定各品系各发育阶段历期和存活率及成虫寿命和单雌产卵量,构建两性生命表。【结果】阿维菌素处理24 h时对中国梨木虱F种群和SS品系3龄若虫的LC_(50)值分别为2.458和0.706 mg/L,F种群、FR品系和FS品系3龄若虫对阿维菌素的抗性倍数分别为3.458,11.965和1.782倍。与FS品系和SS品系相较,FR品系各发育阶段历期和存活率及成虫寿命和单雌产卵量均发生变化,卵历期和若虫历期均显著长于FS品系和SS品系的;成虫寿命缩短,单雌产卵量分别下降57.000和74.300粒,其内禀增长率(r)、周限增长率(λ)和净增殖率(R_(0))也均下降。FR品系相对适合度为0.743,是FS品系的0.807倍。【结论】LC_(50)浓度的阿维菌素连续处理6代使中国梨木虱对阿维菌素的抗性水平增加,适合度下降。因而,在梨园中避免连续使用阿维菌素,以延缓中国梨木虱对阿维菌素的抗性增加。【Aim】To clarify the effects of the medium lethal concentration(LC_(50))of avermectin on the development of short-term resistance of Psylla chinensis to avermectin.【Methods】The LC_(50) value of avermectin to the 3rd instar nymphs of the field population(F)of P.chinensis was determined at 24 h after treatment by leaf-dipping method.Based on the F population,the relative avermectin-sensitive strain(SS),the field resistant strain from the F population after exposure to LC_(50) of avermectin for six consecutive generations(FR)and the field control strain from the F population without exposure to avermectin for six consecutive generations(FS)of P.chinensis were established under indoor conditions.The resistance ratios of the 3rd instar nymphs of the F population and various strains of P.chinensis to avermectin were measured,and the developmental duration and survival rate of each developmental stage,and adult longevity and numbers of eggs laid per female of various strains were measured to construct a two-sex life table.【Results】The LC_(50) values of avermectin to the 3rd instar nymphs of the F population and the SS strain of P.chinensis at 24 h after treatment were 2.458 and 0.706 mg/L,respectively.The resistance ratios of the 3rd instar nymphs of the F population,FR strain and FS strain to avermectin were 3.458,11.965 and 1.782-fold,respectively.The developmental duration and survival rate of each developmental stage,and adult longevity and number of eggs laid per female of the FR strain changed as compared with those of the FS strain and SS strain.The egg duration and nymphal duration of the FR strain were significantly longer than those of the FS strain and SS strain.The adult longevity of the FR strain was shorter than that of the FS strain and SS strain.The number of eggs laid per female of the FR strain was decreased by 57.000 and 74.300 grains,respectively,as compared with those of the FS strain and SS strain.The intrinsic rate of increase(r),finite rate of increase(λ)and net reproductive rate(R_(0

关 键 词：中国梨木虱 阿维菌素 年龄-龄期两性生命表 生长发育 种群参数 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]