不同番茄品种叶片理化性状对南美番茄潜叶蛾寄主选择性的影响  

Effects of physical and chemical characteristics of the leaves of different tomato varieties on the host selectivity of Tuta absoluta(Lepidoptera:Gelechiidae)

作　　者：薛增昇 王韩 张生存 马力 郄杏桃 吕治燊 张东霞 郑卫锋 闫喜中[1] 郝赤[1] XUE Zeng-Sheng;WANG Han;ZHANG Sheng-Cun;MA Li;QIE Xing-Tao;L Zhi-Shen;ZHANG Dong-Xia;ZHENG Wei-Feng;YAN Xi-Zhong;HAO Chi(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China;Shanxi Plant Protection and Plant Quarantine Center,Taiyuan 030001,China)

机构地区：[1]山西农业大学植物保护学院,太谷030801 [2]山西省植物保护植物检疫中心,太原030001

出　　处：《昆虫学报》2023年第12期1590-1601,共12页Acta Entomologica Sinica

基　　金：科技部高端外国专家引进计划(G2022004006L);山西省自然科学基金项目(20210302123387,201901D111231);山西农业大学“特”“优”农业高质量发展科技支撑工程(TYGC-09);山西省研究生教育创新项目(2021Y330)。

摘　　要：【目的】明确南美番茄潜叶蛾Tuta absoluta对不同番茄品种的寄主选择性,分析南美番茄潜叶蛾寄主选择性与不同番茄品种叶片理化性状的相关性,为南美番茄潜叶蛾的绿色精准防控和番茄抗虫品种选育提供理论依据。【方法】用叶碟法测定南美番茄潜叶蛾3龄幼虫对5个番茄品种(千禧、大粉、大黑、小绿和黄矮生)叶片的取食选择率和取食面积,用笼罩法测定南美番茄潜叶蛾成虫在这5个番茄品种每叶片上的产卵量,采用网格法和比色法测定5个番茄品种叶片的理化性状,用Pearson法分析南美番茄潜叶蛾幼虫取食选择率和成虫在每叶片上的产卵量分别与不同番茄品种叶片理化性状的相关性。【结果】寄主选择性结果表明:当南美番茄潜叶蛾3龄幼虫取食6,12,24和48 h时,其对5个番茄品种叶片的取食选择率从大到小均为:大粉>千禧>大黑>小绿>黄矮生。当南美番茄潜叶蛾成虫产卵24,48和72 h时,其在5个番茄品种每叶片上的产卵量从大到小依次为:大黑>千禧>大粉>黄矮生>小绿,大黑>黄矮生>千禧>大粉>小绿,大黑>黄矮生>大粉>千禧>小绿。Pearson相关性分析结果表明:当南美番茄潜叶蛾3龄幼虫取食12 h时,其取食选择率与不同番茄品种叶片蛋白质含量呈显著负相关;当南美番茄潜叶蛾成虫产卵48和72 h时,其每叶片上的产卵量与不同番茄品种叶片叶绿素b含量和总叶绿素含量呈显著正相关。【结论】千禧、大粉、大黑、小绿和黄矮生5个番茄品种叶片理化性状对南美番茄潜叶蛾寄主选择影响显著。根据相关性分析,南美番茄潜叶蛾幼虫更喜欢取食蛋白质含量低的番茄品种叶片,成虫更喜欢在叶绿素含量高的番茄品种叶片上产卵。【Aim】To clarify the host selectivity of Tuta absoluta to different tomato varieties,and analyze the correlations between the host selectivity of T.absoluta and the physical and chemical characteristics of the leaves of different tomato varieties,so as to provide a theoretical basis for the green precise control of T.absoluta and the breeding of insect-resistant tomato varieties.【Methods】The feeding selection rates and consumed area of the 3rd instar larvae of T.absoluta to the leaves of five tomato varieties(Qianxi,Dafen,Dahei,Xiaolv and Huang′aisheng)were measured by leaf disc method,the numbers of eggs laid by T.absoluta adults on per leaf of the five tomato varieties were detected by caging method,the physical and chemical characteristics of the leaves of five tomato varieties were measured by grid method and colorimetric method,and the correlations between the feeding selection rate of the 3rd instar larvae of T.absoluta or the number of eggs laid by female adults on per leaf and the physical and chemical characteristics of the leaves of different tomato varieties were analyzed by Pearson’s method.【Results】The results of host selectivity showed that when T.absoluta larvae fed on the leaves of the five tomato varieties for 6,12,24 and 48 h,their feeding selection rates were ranked in a descending order as follows:Dafen>Qianxi>Dahei>Xiaolv>Huang′aisheng.When T.absoluta adults laid eggs on the leaves of the five tomato varieties for 24,48 and 72 h,their numbers of eggs laid on per leaf were ranked in a descending order as follows:Dahei>Qianxi>Dafen>Huang′aisheng>Xiaolv,Dahei>Huang′aisheng>Qianxi>Dafen>Xiaolv,and Dahei>Huang′aisheng>Dafen>Qianxi>Xiaolv,respectively.The results of Pearson correlation analysis showed that the feeding selection rate of the 3rd instar larvae of T.absoluta was significantly negatively correlated with the protein content in the leaves of different tomato varieties when fed for 12 h.When T.absoluta adults laid eggs for 48 and 72 h,their numbers of eggs laid on pe

关 键 词：南美番茄潜叶蛾 寄主选择 番茄品种 Pearson相关性分析 理化性状 

分 类 号：Q968[生物学—昆虫学]