葱兰夜蛾雌蛾求偶行为节律观察与性信息素鉴定  

Observation of the circadian rhythm of calling behavior and identification of the sex pheromone of female moths of Brithys crini(Lepidoptera:Noctuidae)

作　　者：胡可炎 苑鹏宇 钱沉鱼 贾彩娟 杨文娟 刘春燕 温秀军 马涛 HU Ke-Yan;YUAN Peng-Yu;QIAN Chen-Yu;JIA Cai-Juan;YANG Wen-Juan;LIU Chun-Yan;WEN Xiu-Jun;MA Tao(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Management Office of Wutong Mountain Park,Shenzhen 518004,China;Shenzhen Guangxin Construction Engineering Co.,Ltd.,Shenzhen 518000,China;Forest Resources Conservation Center of Guangdong Province,Guangzhou 510130,China)

机构地区：[1]华南农业大学林学与风景园林学院,广州510642 [2]深圳市梧桐山风景区管理处,深圳518004 [3]深圳市广信建设工程有限公司,深圳518000 [4]广东省森林资源保育中心,广州510130

出　　处：《昆虫学报》2023年第12期1602-1611,共10页Acta Entomologica Sinica

基　　金：广东省科技计划项目(2020A1414010131);校企合作项目(4400-H220365)。

摘　　要：【目的】葱兰夜蛾Brithys crini主要危害石蒜科植物。本研究旨在掌握葱兰夜蛾的求偶行为节律,并鉴定其雌蛾的性信息素组分,为利用性信息素绿色防控葱兰夜蛾提供理论基础和技术指导。【方法】将葱兰夜蛾处女雌蛾单独饲养在塑料碗中,每0.5 h观察1次求偶情况,记录不同日龄每头雌蛾日龄求偶开始和结束时间及求偶时长;并采用重蒸正己烷浸泡法提取处女求偶雌蛾性信息素腺体,利用气相色谱-触角电位联用仪(gas chromatography-electroantennagram detection,GC-EAD)筛选性信息素腺体提取物的活性成分,然后利用气相色谱-质谱联用仪(gas chromatography-mass spectrometry,GC-MS)对活性成分进行分析和滴度(含量)测定,最后利用触角电位(electroatennogram,EAG)仪测试性信息素组分对雄蛾触角的电生理活性。【结果】葱兰夜蛾雌蛾求偶集中在暗期,求偶活动多在暗期3.5-9.0 h,1日龄雌蛾求偶率最高,1-2日龄雌蛾求偶率显著高于3日龄雌蛾。GC-EAD结果显示,葱兰夜蛾性信息素腺体提取物中有1种组分可引起雄蛾的EAG反应;结合GC-MS分析结果和Kov ts保留指数,确定该活性成分为顺-11-十六碳烯醛(Z 11-16∶Ald);通过外标法测得1和2日龄雌蛾性信息素腺体中Z 11-16∶Ald的平均滴度为(3.11±0.01)ng;EAG测试数据显示不同剂量的Z 11-16∶Ald引起葱兰夜蛾雄蛾EAG反应程度不同,当剂量为100 ng时,EAG反应最强烈。【结论】葱兰夜蛾1日龄雌蛾求偶最活跃,求偶多发生暗期3.5-9.0 h,鉴定出性信息素主要组分为Z 11-16∶Ald。【Aim】Brithys crini mainly damage Lycoris plants.The aim of this study is to understand the circadian rhythm of calling behavior of B.crini and identify the sex pheromone components of its female moths,so as to provide the theoretical basis and technical guidance for the application of sex pheromones in green control of B.crini.【Methods】The virgin female moths of B.crini were placed individually in a plastic cup and their calling behavior was observed every 0.5 h.The start and end time of calling,and courtship duration of each female moth at different day-old were recorded.The sex pheromone gland of virgin female moth was extracted using distilled hexane extraction.The active component of sex pheromone gland extracts was screened by gas chromatography-electroantennagram detection(GC-EAD)and gas chromatography-mass spectrometry(GC-MS),and its titer(content)was further determined by GC-MS.The electrophysiological activities of sex pheromone components to the antennae of male moths were measured by electroatennogram(EAG)apparatus.【Results】The peak calling period of the female moths of B.crini was between the 3.5 and 9.0 h into scotophase,and the 1-day-old female moths displayed the highest calling rate.The calling rates of the 1-2-day-old female moths were significantly higher than that of the 3-day-old female adults.GC-EAD results showed that a component of the sex pheromone gland extracts could induce EAG response of male moths.The active component was identified as(Z)-11-hexadecenal(Z 11-16∶Ald)by GC-EAD analysis and Kováts retention index.And the average titer of Z 11-16∶Ald in the sex pheromone glands of the 1-and 2-day-old female moths was(3.11±0.01)ng by external standard method.EAG test data showed that different doses of Z 11-16∶Ald could evoke different EAG responses in male moths of B.crini,and when the dose of Z 11-16∶Ald was 100 ng,the EAG response was the strongest.【Conclusion】The 1-day-old female moths of B.crini show the most active calling behavior,and the peak calling perio

关 键 词：葱兰夜蛾 求偶节律 性信息素 鉴定 顺-11-十六碳烯醛 触角电位(EAG) 

分 类 号：Q968[生物学—昆虫学]