植物激素对烟草花粉萌发及授粉后花粉管生长的影响  

The Influence of Plant Hormones on Nicotiana tabacum Pollen Germination and Pollen Tube Growth after Pollination

作　　者：许杰 魏卓 廖菊够[2] 牛永志 郑昀晔 冯猜 张立猛 陈穗云[2] XU Jie;WEI Zhuo;LIAO Jugou;NIU Yongzhi;ZHENG Yunye;FENG Cai;ZHANG Limeng;CHEN Suiyun(Yuxi Zhongyan Tobacco Seed Company,Yuxi Yunnan 653100,China;School of Ecology and Environmental,Yunnan University,Kunming 650504,China)

机构地区：[1]玉溪中烟种子有限责任公司,云南玉溪653100 [2]云南大学生态与环境学院,昆明650504

出　　处：《种子》2023年第12期59-66,82,共9页Seed

基　　金：玉溪中烟种子有限责任公司科技计划一般项目(2022530401740002);云南省基础研究计划重点项目(202201AS070014);国家自然科学基金项目(32060175和32060043)。

摘　　要：采用离体萌发法研究外源植物激素(GA、IAA、ABA、6-BA)对栽培烟草、花烟草和黄花烟草花粉萌发的影响。结果表明,ABA抑制3个烟草种的花粉萌发;栽培烟草花粉萌发最适的GA浓度为10 mmol/L,IAA浓度为10 mmol/L,6-BA浓度为15 mmol/L。黄花烟草花粉萌发的最适GA浓度为100 mmol/L、IAA浓度为30 mmol/L、6-BA浓度为4 mmol/L。花烟草花粉萌发最适6-BA浓度为1 mmol/L,GA和IAA抑制其花粉萌发。用半离体培养实验探究上述激素对栽培烟草自交,及其作为母本与花烟草和黄花烟草杂交授粉后花粉管生长的影响,结果表明,4种激素均促进花粉管生长,且对栽培烟草(自交亲和)花粉管生长的促进作用最强,但GA抑制花烟草花粉管生长。此外,6-BA还能改善黄花烟草花粉管在杂交授粉过程中的卷曲现象。The influence of exogenous plant hormones GA,IAA,ABA and 6-BA on pollen germination and pollen tube growth after pollination of Nicotiana tabacum L.cv.K326(female parent),N.alata and N.rustica were studied with in vitro culture method.The results showed that ABA inhibited pollen germination of three Nicotiana species.The optimal concentration of both GA and IAA to promote the pollen germination of K326 was 10 mmol/L,and 6-BA was 15 mmol/L on;For pollen of N.rustica,the optimal concentration of GA,IAA and 6-BA were 100 mmol/L,30 mmol/L and 4 mmol/L,respectively.GA and IAA inhibited pollen germination of N.alata,but 1 mmol/L of 6-BA was the optimal concentration to promote its pollen germination.The effects of these hormones on the growth of pollen tubes of Nicotiana tabacum L.cv.K326 after cross-pollination with N.alata and N.rustica as mother were investigated by semi-isolated culture experiment.The results showed that all the four hormones promoted the growth of pollen tubes,and the promotion effect on the growth of pollen tubes of Nicotiana tabacum L.cv.K326(self-compatibility)was the strongest,but GA inhibited the growth of pollen tubes of N.alata.In addition,6-BA could also improve the curling of pollen tubes during cross-pollination.

关 键 词：植物激素 烟草 花粉萌发 花粉管生长 

分 类 号：S572[农业科学—烟草工业]