半乳糖氧化酶的生物学改造研究进展  

Advances in the Biological Modification of Galactose Oxidase

作　　者：夏光丽 曹娜 孙慧慧[2] 赵玲[2] 曹荣[2,3] XIA Guang-li;CAO Na;SUN Hui-hui;ZHAO Ling;CAO Rong(College of Food Science and Engineering,Ocean University of China,Qingdao 266404;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237)

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266404 [2]中国水产科学研究院黄海水产研究所,青岛266071 [3]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,青岛266237

出　　处：《生物技术通报》2024年第1期176-185,共10页Biotechnology Bulletin

摘　　要：半乳糖氧化酶是一种重要的生物催化剂,具有催化效率高和绿色环保的特点,在生物传感器、食品、医疗和化工等领域得到广泛应用。然而,天然的半乳糖氧化酶存在稳定性差、催化效率低、回收成本高等缺陷,限制了其在食品安全和生物医学等领域的应用。随着基因工程和蛋白质工程的发展,人们可以对半乳糖氧化酶进行有目的的改造,以提高酶的性能或者获得新功能的重组酶。因此,追求具有重要催化能力和强大功能的半乳糖氧化酶成为该领域的研究热点。本文综述了半乳糖氧化酶的分子结构、催化机理等基本信息,并分析总结了半乳糖氧化酶进行生物学改造的相关案例,旨在为半乳糖氧化酶相关研究者提供新的思路和方法。As an important biological catalyst,galactose oxidase has the characteristics of high catalytic efficiency and environmental protection.It has been widely used in biosensors,food,medical treatment,chemical industry and other fields.However,natural galactose oxidase has some inherent defects such as poor stability,low catalytic efficiency,and high recovery cost,which greatly limit its application in food safety,biomedicine,and other fields.With the development of genetic engineering and protein engineering,galactose oxidase can be purposefully modified to improve the performance of the enzyme or to obtain recombinant enzymes with new functions.Therefore,the pursuit of galactose oxidase with important catalytic capacity and powerful functions has become a research hotspot in this field.In this paper,the molecular structure and catalytic mechanism of galactose oxidase are reviewed,and the relevant cases of biological modification of galactose oxidase are analyzed and summarized,aiming to provide new ideas and methods for galactose oxidase related researchers.

关 键 词：半乳糖氧化酶 生物学改造 理性设计 定向进化 固定化 

分 类 号：TQ426.97[化学工程]