β-溶血性链球菌对红霉素、克林霉素耐药性及对大环内酯类抗生素耐药机制研究  

STUDY ON RESISTANCE OF BHS TO ERYTHROMYCIN AND CLINDAMYCIN AND MECHANISM OF RESISTANCE OT MACROLIDES

作　　者：程振娜 张凤华[2] 董立 李霞 闫晓华 CHENG Zhen-na;ZHANG Feng-hua;DONG Li;LI Xia;YAN Xiao-hua(Shandong Medical College,Linyi,China 276000;Linyi People's Hospital;Affiliated Hospital of Shandong Medical College)

机构地区：[1]山东医学高等专科学校,山东临沂276000 [2]临沂市人民医院 [3]山东医专附属医院

出　　处：《山东医学高等专科学校学报》2024年第1期1-4,共4页Journal of Shandong Medical College

基　　金：山东省医药卫生科技发展计划项目(No.202101060963)。

摘　　要：目的探讨BHS的耐药性及耐药基因携带情况。方法采用药敏纸片法测定BHS对红霉素、克林霉素的药物敏感性,应用PCR法检测其对大环内酯类药物耐药基因ermB、mefA。结果BHS群对红霉素及林可酰胺类药物克林霉素的耐药率较高。ermB基因阳性率为84.4%,其中GCS、GFS对该基因携带率最高均为100%,7株红霉素敏感菌株中检测出ermB基因,与耐药表型不符。mefA基因阳性率为3.1%,均在红霉素耐药菌株中检出。有1株同时携带ermB、mefA基因。结论BHS群对大环内酯类药物及林可酰胺类药物耐药率较高,其对红霉素耐药主要与核糖体糖蛋白甲基化有关。Objective To explore the drug resistance and resistance gene carrying status of BHS.Methods The drug sensitivity of BHS to erythromycin and clindamycin was determined by the drug sensitivity disk method,and the resistance genes ermB and mefA to macrolides were detected by PCR.Results The resistance rate of BHS group to erythromycin and clindamycin was high.The positive rate of ermB gene was 84.4%,with GCS and GFS having the highest carrieRrate of the gene at 100%.The ermB gene was detected in 7 erythromycin sensitive strains,which did not match the drug resistance phenotype.The positive rate of mefA gene was 3.1%,all of which were detected in erythromycin resistant strains.One strain carries both ermB and mefA genes.Conclusion BHS group has a high resistance rate to macrolides and lincomamides,and its resistance to erythromycin is mainly related to the methylation of ribosome glycoprotein.

关 键 词：Β-溶血性链球菌 红霉素 克林霉素 耐药基因 

分 类 号：R378.12[医药卫生—病原生物学]