维生素C制备大鼠成骨细胞膜片及其活性评估  

Construction of rat osteoblast cell sheet with vitamin C and its activity evaluation

作　　者：杨红 董强 夏茜 周建奖 YANG Hong;DONG Qiang;XIA Qian;ZHOU Jianjiang(Department of Prosthodontics,Guiyang Hospital of Stomatology,Guiyang,Guizhou 550002,China;Department of Prosthodontics,the Affiliated Stomatological Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Oral Medicine,the Affiliated Stomatological Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China;Key Laboratory of Medical Molecular Biology of Guizhou Province,Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]贵阳市口腔医院口腔修复科,贵州贵阳550002 [2]贵州医科大学附属口腔医院口腔修复科,贵州贵阳550004 [3]贵州医科大学附属口腔医院口腔内科,贵州贵阳550004 [4]贵州医科大学贵州省医学分子生物学重点实验室,贵州贵阳550004

出　　处：《医药前沿》2024年第1期20-22,共3页Journal of Frontiers of Medicine

基　　金：国家自然科学基金项目(NS2018-64);贵阳市科技局基金项目(GY2017-43)。

摘　　要：目的:大鼠成骨细胞膜片的体外构建,通过形态学检测及增殖活性检测,评估其活性。方法:原代培养大鼠成骨细胞,纯化鉴定后体外构建成骨细胞膜片,用扫描电镜观察、组织学形态学检查,并测定厚度变化及细胞增殖情况。结果:经过5d培养的成骨细胞连接成片,刮刀可刮下。HE染色见丰富的细胞外基质。扫描电镜观察到细胞层间有间隙孔。与正常培养的成骨细胞相比,细胞膜片第1周至第2周增殖迅速,第2周增殖活力最高。随着培养时间的延长,厚度增加,厚度在1周至2周显著增加,对照组直到3周才形成膜片,且厚度低于维生素C组。结论:维生素C促成骨细胞增殖形成细胞膜片,本方法可以稳定体外构建成骨细胞膜片,2周内膜片厚度增加显著并且增殖活力较好。Objective To construct osteoblast membranes from rat cells in vitro and evaluate their activity through morphological and proliferation assays.Methods Primary cultures of rat osteoblasts were established,purified,and used to construct osteoblast membranes in vitro.The membranes were observed via scanning electron microscopy and subjected to histological morphological examinations.Changes in thickness and cell proliferation were measured.Results Osteoblasts cultured for 5 days formed interconnected membranes detachable by scraping.Hematoxylin and eosin staining revealed abundant extracellular matrix.Scanning electron microscopy showed intercellular gaps.Compared to normally cultured osteoblasts,the proliferation of membrane cells was most rapid between the first and second weeks,peaking in the second week.Thickness notably increased with prolonged culture,significantly rising between the first and second weeks.The control group formed membranes only by the third week,with thickness lower than the Vitamin C group.Conclusions Vitamin C promotes osteoblast proliferation and the formation of cell membranes.This method enables stable in vitro construction of osteoblast membranes,with significant thickness increase within two weeks and good proliferation activity.

关 键 词：细胞膜片 骨组织工程 维生素C 

分 类 号：R473[医药卫生—护理学]