基于高通量测序的鸡粪堆肥过程细菌群落结构分析  

Analysis of bacterial community dynamics in chicken manure composting based on high throughput sequencing

作　　者：张慧[1] 林戎斌[1] 林陈强[1] 贾宪波[1] 苏光秋 陈济琛[1] ZHANG Hui;LIN Rongbin;LIN Chenqiang;JIA Xianbo;SU Guangqiu;CHEN Jichen(Institute of Soil and Fertilizer,Fujian Academy of Agricultural Sciences,Fuzhou 350001,China;Pingnan Agriculture and Rural Bureau,Pingnan 352300,China)

机构地区：[1]福建省农业科学院土壤肥料研究所,福建福州350001 [2]屏南县农业农村局,福建屏南352300

出　　处：《安徽农学通报》2024年第2期83-87,共5页Anhui Agricultural Science Bulletin

基　　金：福建省属公益类竞争性项目(2023R1074);福建省星火项目(2023S0064);福建省农业科学院微生物与土壤安全健康科技创新团队(CXTD2021030)。

摘　　要：为通过相关功能微生物的应用,提高鸡粪堆肥效率及产品质量,本研究以鸡粪和菌渣进行好氧堆肥35 d,利用16S rRNA基因高通量测序技术分析鸡粪堆肥过程中细菌的群落结构动态变化。结果表明,在细菌门分类水平上,鸡粪堆肥过程中主要有厚壁菌门、拟杆菌门和盐厌氧菌门,其中厚壁菌门的相对丰度在鸡粪堆肥各时期均占主导地位,腐熟期相对丰度最高,拟杆菌门在腐熟期相对丰度最低,盐厌氧菌门在降温期相对丰度最高;在细菌纲分类水平上,鸡粪堆肥过程中主要有梭菌纲、芽孢杆菌纲和拟杆菌纲;α多样性相关分析表明,高温期堆肥中细菌菌群丰富度最高,群落多样性最大。综上,在鸡粪堆肥期间,细菌群落结构发生了明显变化。To improve the efficiency and product quality of chicken manure composting through the application of related functional microorganisms,chicken manure and bacterial residue were used for aerobic composting for 35 d,and the dynamic community structure of bacteria in the process of 16S rRNA gene was analyzed.The results showed that Firmicutes,Bacteroidetes and Haloanaerobes were the main types in the composting of chicken manure.The relative abundance of Firmicutes was dominant in all stages of chicken manure composting,and the relative abundance of firmicutes was the highest in the maturing stage,the relative abundance of Bacteroidetes was the lowest in the maturing stage,and the relative abundance of anaerobes salinae was the highest in the cooling stage.On the level of bacteria classification,the main bacteria in chicken manure compost were Clostridium,Bacillus and Bacteroidetes.α-diversity correlation analysis showed that the bacterial community richness and community diversity were the highest in the high temperature compost.The bacterial community structure changed significantly during the composting of chicken manure.

关 键 词：鸡粪 菌渣 好氧堆肥 高通量测序 细菌群落 

分 类 号：S141.4[农业科学—肥料学]