红花龙胆枯萎病病原菌鉴定及培养特性研究  

Identification and biological characteristics of Clarireedia jacksonii causing wilt disease on Gentiana rhodantha in Guizhou

作　　者：严凯 李树江 李敏 张晓勇 YAN Kai;LI Shujiang;LI Min;ZHANG Xiaoyong(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553004,China)

机构地区：[1]六盘水师范学院生物科学与技术学院,六盘水553004

出　　处：《植物保护》2024年第1期63-68,共6页Plant Protection

基　　金：贵州省六盘水师范学院大学科技园(黔科合平台人才DXKJY[2021]003);贵州省特色重点实验室建设项目(黔教合KY字[2017]012);六盘水市科技计划(52020-2018-03-07)。

摘　　要：枯萎病是红花龙胆栽培中发生最普遍,危害最严重的病害之一。为明确引起红花龙胆枯萎病的病原种类及其侵染特性,本研究采用组织分离法获得2个菌株,结合形态学特征以及ITS和Mcm7序列鉴定,2个菌株被鉴定为Clarireedia jacksonii,这是该菌在龙胆科植物上引起枯萎病的首次报道。致病性测定表明,该菌在15~35℃可侵染红花龙胆健康叶片,最佳侵染温度为25℃。生长特性研究表明,该菌最适生长温度为25℃,最适pH为5,菌丝致死温度为50℃处理10 min。Wilt disease is one of the most common and serious diseases of Gentiana rhodantha in the field in Guizhou province.In order to identify the pathogen causing wilt disease on G.rhodantha,two strains were isolated from diseased leaves by tissue separation method.Based on morphological characteristics and analyses of ITS and Mcm7 sequences,the pathogen was identified as Clarireedia jacksonii.This is the first report of C.jacksonii causing wilt disease on Gentianaceae plants.Pathogenicity test showed that the pathogen could infect the healthy leaves at 15-35℃,and the optimal infection temperature was 25℃.In culture tests,the optimum conditions for mycelial growth were 25℃ and pH 5,and the lethal temperature for mycelium was 50℃ for 10 min.

关 键 词：红花龙胆 枯萎病 病原菌鉴定 侵染特性 生长特性 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]