青蒿中番茄斑萎病毒和烟草花叶病毒的分子鉴定及相关序列分析  

Molecular identification and sequence analysis of tomato spotted wilt virus and tobacco mosaic virus on Artemisia caruifolia

作　　者：钟静[1] 李婷婷[1] 韩天华 李学卫 尹跃艳[3] 陈越[1] 马文广 丁铭[1] ZHONG Jing;LI Tingting;HAN Tianhua;LI Xuewei;YIN Yueyan;CHEN Yue;MA Wenguang;DING Ming(Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Yunnan Provincial Key Laboratory of Agricultural Biotechnology,Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming 650223,China;Lijiang Branch Company of Yunnan Tobacco Company,Lijiang 674100,China;Institute of Alpine Economic Plant,Yunnan Academy of Agricultural Sciences,Lijiang 674199,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,农业农村部西南农业基因资源与种质创制重点实验室,昆明650223 [2]云南省烟草公司丽江市公司,丽江674100 [3]云南省农业科学院高山经济植物研究所,丽江674199

出　　处：《植物保护》2024年第1期244-255,共12页Plant Protection

基　　金：中国烟草总公司云南省公司科技计划(2021530000242016);云南省种子种业联合实验室项目(202205AR070001-02);国家自然科学基金(32160619)。

摘　　要：番茄斑萎病毒(tomato spotted wilt virus, TSWV)和烟草花叶病毒(tobacco mosaic virus, TMV)是2种重要的植物病原病毒,对多种经济作物的产量和品质均造成严重影响。2021年-2022年,在云南省丽江市烟草种植区不同烟区采集叶片黄化、皱缩以及无症状的青蒿Artemisia caruifolia样品共计14份,利用免疫金标速测卡和RT-PCR对其病原病毒进行检测。利用免疫金标速测卡检测结果显示,在所检样品中有9份样品检测出TSWV,检出率为64.28%,有3份样品检测出TMV,检出率为21.43%,2种病毒复合侵染的检出率同样为21.43%;利用RT-PCR对复合侵染的3份样品进行分子检测,结果显示,在3份复合侵染青蒿样品中获得3条TSWV N基因序列、3条TMV cp基因序列和2条TMV RdRp部分序列。TSWV青蒿分离物与分离自云南的TSWV-2分离物相似性最高,为99.6%;TMV青蒿分离物与分离自辽宁的TMV-Shenyang分离物和分离自云南的TMV-Yongren-1相似性最高,均大于99.4%。这是首次发现TSWV和TMV 2种不同属病毒复合侵染青蒿。Tomato spotted wilt virus(TSWV)and tobacco mosaic virus(TMV)are two important viral pathogens that account for significant yield losses and quality damage in many economically important crops.During 2021-2022,14 sweet wormwood(Artemisia caruifolia)leaf samples with leaf yellowing and curling symptoms or asymptomatic were collected from tobacco fields in Lijiang of Yunnan province.The colloidal gold antigen rapid screen kits and RT-PCR were used to identify the pathogens of diseased plants.Immunological assays using colloidal gold antigen rapid screen kits showed that nine leaf samples were infected with TSWV(64.28% positive rate),three infected with TMV(21.43%positive rate),and three infected with both TSWV and TMV.RT-PCR was carried out to obtain viral sequences from these three co-infected sweet wormwood samples.Then three N gene sequences of TSWV,three cp sequences and two partial RdRp sequences of TMV were obtained.Sequence alignment showed that the TSWV isolates were most closely related to the TSWV-2 isolate from Yunnan sharing 99.6%sequence identity,and the TMV isolates shared the highest sequence identity(>99.4%)with the Shenyang isolate from Liaoning and Yongren-1 isolate from Yunnan.It is the first report that sweet wormwood plants were co-infected by TSWV and TMV.

关 键 词：番茄斑萎病毒 烟草花叶病毒 青蒿 复合侵染 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]