薏苡仁油联合5-FU对结肠癌HCT-116细胞株增殖、凋亡的影响  

Influence of Coix Seed Oil Jointed with 5-FU on the Proliferation and Apoptosis of Colon Cancer HCT-116 Cell Line

作　　者：王韬[1] 费建东[1] 聂双发[1] 李磊[1] WANG Tao;FEI Jiandong;NIE Shuangfa;LI Lei(Department of Gastrointestinal Surgery,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院胃肠外科,河北张家口075000

出　　处：《西部中医药》2024年第1期9-12,共4页Western Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局2019年度中医药类科研课题(2019180);河北省卫计委2018年度医学科学研究重点课题(20180821)。

摘　　要：目的:分析薏苡仁油联合5-氟尿嘧啶(5-fluorouracil,5-FU)对结肠癌HCT-116细胞株增殖、凋亡的影响及其作用机制。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法测定不同浓度薏苡仁油、5-FU以及薏苡仁油+5-FU对人结肠癌HCT-116细胞株的生长抑制效应;采用流式细胞术检测细胞凋亡情况;采用逆转录聚合酶链反应(RT-PCR)检测生存蛋白(Survivin)mRNA表达水平。结果:薏苡仁油、5-FU、5-FU+薏苡仁油均对人结肠癌HCT-116细胞增殖产生一定抑制作用,且薏苡仁油浓度越高,作用时间越长,细胞增殖抑制率越高,3组比较差异有统计学意义(P<0.05);与薏苡仁油组及5-FU组相比,5-FU+薏苡仁油组细胞增殖抑制率更高,差异有统计学意义(P<0.05)。薏苡仁油组、5-FU组、5-FU+薏苡仁油组的细胞凋亡率均高于空白对照组,差异有统计学意义(P<0.05);薏苡仁油浓度越高,细胞凋亡率也越高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组细胞凋亡率高于5-FU组及薏苡仁油组,差异有统计学意义(P<0.05)。与空白对照组相比,不同浓度薏苡仁油组Survivin mRNA表达降低,差异有统计学意义(P<0.05);5-FU组Survivin mRNA表达升高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组Survivin mRNA表达较5-FU组降低,差异有统计学意义(P<0.05)。结论:薏苡仁油能抑制结肠癌HCT-116细胞增殖,诱导细胞凋亡,与5-FU联合应用能发挥药物协同作用,其作用机制可能与调控Survivin mRNA表达有关。Objective:To analyze the effects of coix seed oil and 5-FU on the proliferation and apoptosis of colon cancer HCT-116 cell line,and its mechanism.Methods:The inhibitory effects of coix seed oil for injection with different concentrations,5-FU and coix seed oil+5-FU on the growth of human colon cancer HCT-116 cell line were detected by using MTT method;cellular apoptosis conditions were measured through using flow cytometry;the expression of Survivin was checked by adopting RT-PCR.Results:Coix seed oil for injection,5-FU and coix seed oil for injection+5-FU produced certain inhibitory effects on the proliferation of human colon cancer HCT-116,the higher the concentration of coix seed oil for injection was,the longer the action time was,the higher cellular proliferation inhibitory rate was,the difference had statistical meaning between three groups(P<0.05);compared with coix seed oil for injection group and 5-FU group,cellular proliferation inhibitory rate was higher in the group of coix seed oil for injection+5-FU,and the difference showed statistical meaning(P<0.05).Cellular apoptosis rates of coix seed oil for injection group,5-FU group and the group of coix seed oil for injection+5-FU were higher than that of blank control group,and the difference was statistically significant(P<0.05);the higher the concentration of coix seed oil for injection was,the higher the apoptosis rate was,the difference had statistical meaning(P<0.05);cellular apoptosis rate in the group of coix seed oil for injection+5-FU was higher than that of 5-FU group and coix seed oil for injection group,and the difference had statistical meaning(P<0.05).the compared with blank control group,expressions Survivin mRNA lowered in coix seed oil for injection group,and the difference had statistical meaning(P<0.05);the expressions of Survivin mRNA in 5-FU group rose,and the difference showed statistical meaning(P<0.05);the expressions of Survivin mRNA in 5-FU+coix seed oil for injection group were lower than these of 5-FU group,and the difference wa

关 键 词：结肠癌 增殖 凋亡 薏苡仁油 5-氟尿嘧啶 

分 类 号：R256.4[医药卫生—中医内科学]