氟吡菌酰胺和吡唑醚菌酯在葡萄上的残留及膳食风险评估  

Residues and dietary risk assessment of fluopyram and pyraclostrobin in grape

作　　者：欧将 梁骥[1,2] 宋强勇 晏姣[1,2] 杨志富 熊锋 陈力华 欧晓明 OU Jiang;LIANG Ji;SONG Qiangyong;YAN Jiao;YANG Zhifu;XIONG Feng;CHEN Lihua;OU Xiaoming(National Engineering Research Center for Agrochemicals/Hunan Province Key Laboratory for Agrochemicals,Hunan Research Institute of Chemical Industry,Changsha 410014,China;Hunan Safety Science and Technology Co.,Ltd.,Changsha 410014,China)

机构地区：[1]湖南化工研究院国家农药创制工程技术研究中心/湖南省农用化学品重点实验室,长沙410014 [2]湖南安全生产科学研究有限公司,长沙410014

出　　处：《农业环境科学学报》2024年第1期30-36,共7页Journal of Agro-Environment Science

摘　　要：为明确氟吡菌酰胺和吡唑醚菌酯在葡萄上的残留风险,开展了一年十地的规范残留试验,建立了高效液相色谱法(HPLC)测定葡萄中氟吡菌酰胺和吡唑醚菌酯残留的分析方法。结果表明,在0.1~20.0 mg·L^(-1)范围内,氟吡菌酰胺和吡唑醚菌酯质量浓度与其峰面积均呈良好的线性关系,相关系数大于0.99。在0.05、0.50 mg·kg^(-1)和2.00 mg·kg^(-1)添加水平下,氟吡菌酰胺和吡唑醚菌酯在葡萄中的平均回收率分别为88.1%~112.3%和90.5%~101.5%,相对标准偏差(RSD)分别为3.1%~8.2%和3.5%~9.1%,检测方法定量限(LOQ)均为0.05 mg·kg^(-1)。采收间隔期为14 d,时葡萄中氟吡菌酰胺和吡唑醚菌酯的残留中值(STMR)分别为0.16 mg·kg^(-1)和0.12 mg·kg^(-1),最高残留值(HR)分别为1.44 mg·kg^(-1)和1.48 mg·kg^(-1)。葡萄中氟吡菌酰胺和吡唑醚菌酯对一般人群的长期膳食摄入风险的贡献率分别为0.142%和0.036%,短期膳食摄入风险商分别为7.8%和5.7%,对1~6岁儿童的短期膳食摄入风险商分别为19.6%和14.4%。研究表明,按照推荐剂量规范使用,氟吡菌酰胺和吡唑醚菌酯在葡萄中的残留量对1~6岁儿童和一般人群造成的健康风险处于安全水平。To determine the residue risks of fluopyram and pyraclostrobin on grapes,the supervised residue trials were conducted in 10 regions within 1 year.A highly efficient and simple method was developed for the determination of fluopyram and pyraclostrobin in grapes using high-performance liquid chromatography(HPLC).The results demonstrated that a good linear relationship between the peak area and the concentration of fluopyram and pyraclostrobin was observed in the range of 0.1-20.0 mg·L^(-1)with a correlation coefficient greater than 0.99.The average recoveries of fluopyram and pyraclostrobin in grapes at three spiked levels of 0.05,0.50,and 2.00 mg·kg^(-1)were 88.1%^(-1)12.3%and 90.5%^(-1)01.5%,with the relative standard deviations(RSDs)ranged from 3.1%-8.2%and 3.5%-9.1%,respectively.The limits of quantification(LOQs)of the two analytes in grape were all 0.05 mg·kg^(-1).The supervised trial median residues(STMR)of fluopyram and pyraclostrobin in grapes were 0.16 and 0.12 mg·kg^(-1),and the highest residues(HR)were 1.44 and 1.48 mg·kg^(-1),respectively,at 14 d after the last application.According to the dietary risk assessment,the contribution rates of fluopyram and pyraclostrobin in grapes to the long-term dietary risk of the general population were 0.142%and 0.036%,the short-term dietary intake risk quotients were 7.8%and 5.7%,and the short-term dietary intake risk quotients for children aged 1-6 years were 19.6%and 14.4%,respectively.These results show that the residues of fluopyram and pyraclostrobin in grapes would not pose significant health risks to children aged 1-6 years and the general population when fluopyram and pyraclostrobin formulation are applied at the recommended dose.

关 键 词：氟吡菌酰胺 吡唑醚菌酯 葡萄 残留 膳食风险评估 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]