Nlrp3基因敲除小鼠听性脑干反应的分析  

Auditory Brainstem Responses in Nlrp3 Gene Knockout Mice

作　　者：周莹 王刚 屈昌北 芦文俊 王磊 孙喆喆 朱莹蕾 张艺俨 吴玮 ZHOU Ying;WANG Gang;QU Changbei;LU Wenjun;WANG Lei;SUN Zhezhe;ZHU Yinglei;ZHANG Yiyan;WU Wei(Department of otolaryngology,Strategic Support Force Medical Center,Beijing 100101,China;State Environmental Protection Key Laboratory of Environmental Sense Organ Stress and Health,Beijing 100101,China)

机构地区：[1]中国人民解放军战略支援部队特色医学中心耳鼻喉科,北京100101 [2]国家环境保护环境感官应激与健康重点实验室,北京100101

出　　处：《中华耳科学杂志》2023年第5期671-676,共6页Chinese Journal of Otology

基　　金：战略支援部队特色医学中心项目(19ZX23)。

摘　　要：目的观察不同月龄Nlrp3基因敲除小鼠听功能特点。方法32只Nlrp3基因敲除的纯合子小鼠与32只C57野生型小鼠根据月龄分成4组:1月、3月、6月、12月组,分别行听性脑干反应(ABR)检测,刺激声用click及tone burst(16kHz),计数耳蜗毛细胞的数目。结果Nlrp3基因敲除纯合子小鼠1月、3月、6月、12月组click刺激ABR听阈分别为:(41.670±12.800)dB,(44.380±17.309)dB,(45.000±16.298)dB,(60.000±6.200)dB。野生型小鼠不同月龄的click-ABR平均听阈分别为:(19.500±8.790)dB,(23.750±11.790)dB,(25.940±2.016)dB,(41.670±4.710)dB。经统计学分析,除了3月和6月龄的Nlrp3-/-小鼠的听阈两者比较P>0.05,差异无统计学意义,其他各组间两者比较P<0.05,差异有统计学意义。Tone burst(16k)结果趋势与click刺激ABR听阈结果基本一致。Nlrp3-/-小鼠基底膜外毛细胞部分缺失,数目低于C57小鼠。结论Nlrp3基因敲除后小鼠耳蜗外毛细胞数量减少,听力中重度下降,并且随着月龄增加而听力下降加重,在12月龄时听力出现明显下降。Nlrp3是参与感音神经性耳聋或老年性聋发生的因素之一。Objective To evaluate hearing in Nlrp3 gene knockout mice at different months of age.Methods Thirty-two Nlrp3 knockout homozygous mice and 32 wild-type C57 mice were divided into 4 groups based on age,i.e.1,3,6 and 12 months.Auditory brain stem responses(ABRs)were detected using clicks and tone bursts(16 kHz),and outer hair cells were counted.Results In homozygous Nlrp3 knockout mice,ABR thresholds were 41.670±12.800 dB SPL,44.380±17.309 dB SPL,45.000±16.298 dB SPL and 60.000±6.200 dB SPL at 1,3,6 and 12 months,respectively,compared to 19.500±8.790 dB SPL,23.750±11.790 dB SPL,25.940±2.016 dB SPL and 41.670±4.710 dB SPL in wild-type mice,with threshold differences at 1 and 12 months being statistically significant(P<0.05).ABR results with tone burst(16k)were similar.Partial loss of outer hair cells was noticed in Nlrp3-/-mice,with less outer hair cells than in C57 mice.Conclusion Nlrp3 gene knockout leads to decreased number of outer hair cells and moderate to severe hearing loss in mice.Hearing deteriorated with age,especially at 12 months.Nlrp3 is one of the factors involved in sensorineural hearing loss and senile hearing loss.

关 键 词：小鼠 听性脑干反应 反应阈 NLRP3 感音神经性听力损失 

分 类 号：R764[医药卫生—耳鼻咽喉科]