紫苜蓿提取物抵抗光损伤的作用及其机理研究  

Mechanism of the Medicago Sativa Extract Against Ultraviolet and Blue Light Induced Photodamage

作　　者：黄芳 周利丹 卢伊娜 HUANG Fang;ZHOU Lidan;LU Yina(JAKA BIOTECH Co.,LTD.,Shanghai 200241,China)

机构地区：[1]上海珈凯生物股份有限公司,上海200241

出　　处：《中国美容医学》2024年第1期58-62,共5页Chinese Journal of Aesthetic Medicine

摘　　要：目的:通过多种评价方法,探究紫苜蓿提取物(Medicago Sativa extract,MS)抵抗光损伤的作用及其机理。方法:通过体外清除ABTS自由基模型,高频蓝光(High-energy visible light,HEVL)照射角质形成细胞(HaCaT)产生ROS自由基模型,紫外线照射HaCaT后细胞活力、产生前列腺素E2(Prostaglandin E_(2),PGE_(2))和基质金属蛋白酶1(Matrix metalloproteinase 1,MMP-1)模型及HEVL诱导的人体皮肤损伤模型来验证MS的抗光损伤功效。结果:MS具有良好的抗氧化能力,其清除ABTS自由基的IC50为0.048%,质量分数为0.25%~0.5%的MS可显著减少HEVL照射下的HaCaT细胞中ROS活性氧的产生,质量分数为0.1%~0.3%的MS剂量依赖性地增加紫外线辐照损伤模型中细胞存活率,并降低PGE_(2)和MMP-1的表达量。在HEVL诱导的人体皮肤损伤模型中,质量分数为0.5%~5%的MS能明显降低皮肤红斑的形成,增加皮肤亮度,减少黑色素的生成。结论:MS作为一种植物来源的抗光损伤型原料,可应用于舒缓、修护及抗皱类功效宣称的产品中。Objective To explore the effect and mechanism of Medicago Sativa extract(MS)against light damage through various evaluation methods.Methods The ABTS free radical detection model was built in vitro,the ROS free radical detection model was produced by high energy visible light(HEVL)irradiation on keratinocytes(HaCaT),the cell viability,production of prostaglandin E_(2)(PGE_(2))and matrix metalloproteinase-1(MMP-1)models after UV irradiation on HaCaT,and the human skin injury model induced by HEVL were used to verify the efficacy of MS.Results MS had a good antioxidant capacity.The IC50 of MS scavenging ABTS free radicals was 0.048%.MS with a mass fraction of 0.25%-0.5%could significantly reduce ROS production in HaCaT cells exposed to HEVL.MS with a mass fraction of 0.1%-0.3%increased the cell survival rate in UV irradiation injury model in a dose-dependent manner,and decreased the expression of prostaglandin E_(2)(PGE_(2))and matrix metalloproteinase-1(MMP-1).In the human skin injury model induced by HEVL,MS with a mass fraction of 0.5%-5%can significantly reduce the formation of skin erythema,increase skin brightness and reduce the formation of melanin.Conclusion As a kind of anti photodamage raw material from plants,MS can be used in the products claimed to have soothing,repairing and anti wrinkle effects.

关 键 词：紫苜蓿提取物 光损伤 抗氧化 高频蓝光 紫外线 抗皱 

分 类 号：R285[医药卫生—中药学]