益肾泄浊方对肾纤维化大鼠的治疗作用及机制  

Therapeutic effect and its mechanism of Yishen Xiezhuo decoction on renal fibrosis in rats

作　　者：胡超娜 高继宁 HU Chaona;GAO Jining(Department of Nephrology,Third Clinical College,Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,China;Department of Nephrology,Shanxi Provincial Hospital of Integrated Traditional Chinese and Western Medicine)

机构地区：[1]山西中医药大学第三临床学院肾病教研室,太原030024 [2]山西省中西医结合医院肾病科

出　　处：《山西医科大学学报》2024年第1期57-64,共8页Journal of Shanxi Medical University

基　　金：山西省卫生健康委科研项目(2023095);山西中医药大学科技创新能力培训计划项目(2021PY-JC-02);山西省中医药管理局科研课题项目(2023ZYYB022)。

摘　　要：目的探讨益肾泄浊方对腺嘌呤所致的肾纤维化大鼠的作用及其机制。方法66只大鼠随机分为空白组、模型组、尿毒清组(1.6 g/kg)、益肾泻浊方低剂量组(0.65 g/kg)、益肾泻浊方中剂量组(1.3 g/kg)、益肾泻浊方高剂量组(2.6 g/kg),每组各11只。除空白组外,其余各组采用腺嘌呤(200 mg/kg)灌胃28 d诱导肾纤维化大鼠模型,造模后,尿毒清组和益肾泄浊方组给药治疗8周。测定SCr和BUN含量、24 h UTP和24 h mAlb水平;HE和Masson染色镜下观察肾脏组织病理变化;免疫组化测大鼠肾脏组织分泌型糖蛋白(Wnt1)、β-连环蛋白(β-catenin)的蛋白表达情况。结果与空白组比较,模型组、尿毒清组、低剂量组、中剂量组和高剂量组24 h UTP和24 h mAlb、SCr和BUN含量、Wnt1和β-catenin蛋白表达均升高(P<0.05);与模型组比较,尿毒清组、低剂量组、中剂量组和高剂量组24 h UTP和24 h mAlb、SCr和BUN水平、Wnt1和β-catenin蛋白表达均降低(P<0.05);与尿毒清组比较,低剂量组24 h UTP和24 h mAlb、SCr和BUN水平差异无统计学意义(P>0.05),Wnt1和β-catenin蛋白表达均降低(P<0.05),中剂量组和高剂量组24 h UTP和24 h mAlb、SCr和BUN水平、Wnt1和β-catenin蛋白表达均降低(P<0.05);与低剂量组比较,中剂量组和高剂量组24 h UTP和24 h mAlb、SCr和BUN水平、Wnt1和β-catenin蛋白表达均降低(P<0.05);与中剂量组比较,高剂量组24 h UTP和24 h mAlb、SCr和BUN水平、Wnt1和β-catenin蛋白表达均降低(P<0.05)。结论益肾泄浊方能够通过调控Wnt/β-catenin信号通路来阻抑肾纤维化的发展,从而起到保护肾脏的作用。Objective To observe the regulatory effect of Yishen Xiezhuo decoction on adenine-induced renal fibrosis in rats and explore its underlying mechanisms.Methods Sixty-six rats were randomly divided into blank group,model group,Niaoduqing group(1.6 g/kg),low dose group(0.65 g/kg Yishen Xiezhuo decoction),medium dose group(1.3 g/kg Yishen Xiezhuo decoction),and high dose group(2.6 g/kg Yishen Xiezhuo decoction),with 11 rats in each group.Except for blank group,the rats in the other groups were orally administrated with adenine(200 mg/kg)to induce the renal fibrosis for 28 d.After modeling,the rats in Niaoduqing group and Yishen Xiezhuo decoction groups were treated with medication for 8 weeks.The levels of SCr and BUN,24 h UTP and 24 h mAlb were determined.Pathological changes of renal tissues were observed by HE and Masson staining.Immunohistochemistry was performed to detect the protein expression of secreted frizzled-related protein(Wnt1)and β-catenin in rat renal tissues.Results Compared with blank group,the levels of 24 h UTP,24 h mAlb,SCr and BUN,and the expressions of Wnt1 and β-catenin proteins were increased in model group,Niaoduqing group,low dose group,medium dose group and high dose group(P<0.05).Compared with model group,the levels of 24 h UTP,24 h mAlb,SCr and BUN,and the expressions of Wnt1 and β-catenin proteins were decreased in Niaoduqing group,low dose group,medium dose group and high dose group(P<0.05).Compared with Niaoduqing group,24 h UTP and 24 h mAlb,SCr and BUN levels showed no significant changes in low dose group(P>0.05),but the expressions of Wnt1 and β-catenin proteins were decreased(P<0.05).Compared with Niaoduqing group,the levels of 24 h UTP,24 h mAlb,SCr and BUN,and the expressions of Wnt1 and β-catenin proteins were decreased in medium dose group and high dose group(P<0.05).Compared with low dose group,the levels of 24 h UTP,24 h mAlb,SCr and BUN,and the expressions of Wnt1 andβ-catenin proteins were decreased in medium dose group and high dose group(P<0.05).Compared with medi

关 键 词：益肾泄浊方 腺嘌呤 肾纤维化 WNT/Β-CATENIN信号通路 慢性肾脏病 

分 类 号：R692[医药卫生—泌尿科学]