川崎病小鼠模型的超声心动图评估  

Echocardiography assessment of Kawasaki disease mouse model

作　　者：张雪梅[1] 郝睿 焦富勇[3] 娄萍 张丹[1] 赵欣[1] 曹玲 赵颖[1] 王俊香 ZHANG Xuemei;HAO Rui;JIAO Fuyong;LOU Ping;ZHANG Dan;ZHAO Xin;CAO Ling;ZHAO Ying;WANG Junxiang(Ultrasound Diagnosis Center,Shaanxi Provincial People's Hospital,Xi'an 710068,China;Department of Ultrasound,Ninth Hospital of Xi'an;Department of Pediatrics,Children's Hospital,Shaanxi Provincial People's Hospital)

机构地区：[1]陕西省人民医院超声诊断中心,西安710068 [2]西安市第九医院超声医学科 [3]陕西省人民医院儿童病院儿科

出　　处：《山西医科大学学报》2024年第1期71-75,共5页Journal of Shanxi Medical University

基　　金：陕西省国际科技合作计划项目(2021KW-66);陕西省重点研发计划项目(2023-YBSF-590)。

摘　　要：目的建立干酪乳杆菌细胞壁成分(LCWE)诱导的川崎病(KD)小鼠模型,利用连续高分辨率小动物心脏超声观察该模型冠状动脉成像及心功能变化,为KD小鼠动物模型的制备及其功能评价提供依据。方法将50只小鼠随机分为两组:模型组(使用LCWE诱导KD小鼠模型,n=30)及对照组(无任何干预,n=20)。两组分别于造模后第15,21,30天使用小动物超声诊断仪测量冠状动脉内径,M型超声记录左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)、左心室射血分数(LVEF)及左心室短轴缩短率(LVFS)。对照组及造模后第15,21,30天模型组分别处死1只小鼠并取出心脏标本进行病理检查。结果造模后第15,21天模型组超声心动图显示冠状动脉管壁与其周围出现高回声;造模后第30天模型组冠状动脉管壁弥漫性增厚。造模后第21天模型组左右冠状动脉内径均大于对照组(P<0.05);造模后第15和30天模型组左右冠状动脉内径与对照组比较无统计学差异(P<0.05)。造模后第15天模型组LVEDD、LVESD、LVEF及LVFS均低于对照组(P<0.05);造模后第21天模型组LVEF及LVFS低于对照组(P<0.05);造模后第30天模型组LVEDD、LVEF、LVFS低于对照组(P<0.05)。病理结果显示,造模后第15天小鼠心外膜间质水肿,少量淋巴细胞浸润及纤维结缔组织弥漫轻度增生,局部心肌纤维结缔组织增生并伴有固体钙盐沉着;造模后第21天小鼠心外膜局部可见多量淋巴细胞、嗜酸粒细胞、单核细胞浸润;造模后第30天小鼠冠状动脉管壁增厚,弹力纤维不连续,内膜轻度增厚,局部玻璃样变性。结论LCWE诱导KD冠脉损伤与自然病程状态的患儿损伤情况相似,可以用来模拟KD冠状动脉损伤及心功能的变化过程。Objective To observe the changes of coronary artery imaging and cardiac function in the murine model of LCWE-induced Kawasaki disease(KD)using high frequency ultrasound system and provide a basis for the preparation of murine KD model and its function assessment.Methods Fifty mice were randomly divided into two groups:model group(LCWE-induced KD model,n=30)and control group(no treatment,n=20).The coronary artery dimensions were measured at day 15,21 and 30 after modeling by small animal ultrasound,left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD),left ventricular ejection fraction(LVEF)and left ventricular short axis shortening rate(LVFS)were recorded by M-mode echocardiography.One mouse in control group and model group was killed at day 15,21,30 to collect the heart samples for pathological examination,respectively.Results Echocardiography showed a high density echo around the coronary artery wall in model group at day 15,21 after modeling,and diffuse thickening at day 30 after modeling.The diameters of both left and right coronary arteries in model group were larger than those in control group at day 21(P<0.05);however,there was no significant difference in the inner diameter of coronary artery between two groups at day 15 and 30 after modeling(P>0.05).LVEDD,LVESD,LVEF and LVFS were lower in model group than in control group at day 15 after modeling(P<0.05),and LVEF and LVFS were lower at day 21(P<0.05),and LVEDD,LVEF and LVFS were lower at day 30(P<0.05).At day 15 after modeling,the interstitial edema of heart,mild lymphocyte infiltration and diffuse mild hyperplasia of fibrous connective tissue on the epicardium were found,and the myocardial hyperplasia accompanied by calcium deposition was also detected.At day 21 after modeling,the epicardium of KD mouse was found to be infiltrated by a large number of lymphocytes,eosinophils and monocytes.At day 30 after modeling,the coronary artery of KD mouse presented thickened wall,discontinuous elastic fibers,slightly thickene

关 键 词：川崎病 超声心动图 心功能 冠状动脉损伤 小鼠 干酪乳杆菌细胞壁成分 

分 类 号：R725[医药卫生—儿科]