重组人源Ⅲ型胶原蛋白对豚鼠紫外线辐射损伤皮肤的修复效果探究  

Study on the Repairing Effect of Recombinant Human TypeⅢCollagen on Guinea Pig Skin Damaged by Ultraviolet Radiation

作　　者：刁立琴 李华 施麟 于月欣 谢媛 王亚如 徐兰举 DIAO Liqin;LI Hua;SHI Lin;YU Yuexin;XIE Yuan;WANG Yaru;XU Lanju(Hebei Institute for Drug and Medical Device Control,Shijiazhuang 050227,China;Hebei NACOL Biotechnology Co.,Ltd.,Shijiazhuang 050000,China)

机构地区：[1]河北省药品医疗器械检验研究院,石家庄050227 [2]河北纳科生物科技有限公司,石家庄050000

出　　处：《生物技术进展》2024年第1期48-54,共7页Current Biotechnology

基　　金：河北省药品监督管理局科技计划项目(2022ZC1021)。

摘　　要：由于大气臭氧层逐渐被破坏,紫外线辐射量大大增加,导致皮肤中的核酸、蛋白质等大分子物质发生化学反应,引起皮肤红斑、色素沉积、晒伤、光老化甚至癌变的发生。以重组人源Ⅲ型胶原蛋白(recombinant human typeⅢcollagen,rhColⅢ)为研究对象,探究其对成纤维细胞(L929)的细胞毒性及对豚鼠紫外线辐射光毒性损伤皮肤的修复作用。结果发现,在体外细胞实验中rhColⅢ对L929细胞无潜在的细胞毒性;体内动物实验中rhColⅢ对豚鼠紫外线辐射光毒性损伤皮肤具有明显的修复作用,其可以减少豚鼠皮肤损伤后的皮肤皱纹,改善损伤皮肤组织的表皮增生和真皮层炎症反应,降低波形蛋白(Vimentin)和黑色素瘤单克隆抗体45(human melanoma black 45,HMB45)在损伤皮肤组织中的表达。结果表明,rhColⅢ的安全性良好,在豚鼠紫外线辐射损伤皮肤的修复治疗中,可以明显改善紫外辐射豚鼠皮肤的表皮层增厚和角质化程度,降低真皮层处的炎症反应,有效调节成纤维细胞纤维化程度与黑色素瘤的发病风险。结果表明,rhColⅢ对紫外线辐射造成的光损伤皮肤具有明显的治疗效果。Due to the gradual destruction of the ozone layer,the amount of ultraviolet radiation is greatly increased,which would lead to chemical reactions in the skin of nucleic acids,proteins and other large molecules,resulting in skin erythema,pigmenta⁃tion,sunburn,photoaging and even cancer.Taking recombinant human typeⅢcollagen(rhColⅢ)as the research object,the cytotoxicity of recombinant human typeⅢcollagen on fibroblasts(L929)and its repairing effect on skin damaged by ultraviolet radiation in guinea pigs were investigated.The results showed that rhColⅢhad no potential cytotoxicity to L929 cells in vitro.In vivo animal experiments have confirmed that rhColⅢhad obvious repairing effect on the skin damaged by ultraviolet radiation phototoxicity in guinea pigs,which could reduce the skin wrinkles after skin injury,improved the epidermal hyperplasia of the injured skin tissue and the inflammation of the dermis.The expression of Vimentin and human melanoma monoclonal antibody 45(HMB45)in injured skin were decreased.The above results indicated that rhColⅢhad good safety in the repair treatment of the skin damaged by ultraviolet radiation in guinea pigs,rhColⅢcould significantly improve the degree of epidermal layer thicken⁃ing and keratinization of the skin of guinea pigs exposed to ultraviolet radiation,reduced the inflammatory response in the dermis and effectively regulate the degree of fibroblast fibrosisand the risk of melanoma.Results showed that,rhColⅢraw material has obvious therapeutic effect on skin damage caused by ultraviolet radiation.

关 键 词：重组人源Ⅲ型胶原蛋白 紫外辐射 光毒损伤 皮肤修复效果 

分 类 号：Q291[生物学—细胞生物学] R751[医药卫生—皮肤病学与性病学]