生物检测试剂保藏技术研究进展  

Research Progress of Biological Detection Reagent Preservation Technology

作　　者：刘莹莹 赵可心 王艺霏 路凯旋 胡孔新[1] LIU Yingying;ZHAO Kexin;WANG Yifei;LU Kaixuan;HU Kongxin(Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《生物技术进展》2024年第1期94-101,共8页Current Biotechnology

基　　金：国家重点研发计划项目(2021YFC2600503);北京市科技计划项目(Z221100005222007)。

摘　　要：生物检测试剂的扩增性能,尤其是酶试剂的生物活性对荧光定量PCR等的检测结果起决定性作用。采用不同的保藏技术对维持检测试剂的生物活性,实现生物检测试剂室温条件下长时间稳定保藏与非冷链运输,保证检测结果的稳定性、准确性、特异性和灵敏度等至关重要。针对低温保藏技术、真空冷冻干燥技术、喷雾干燥技术以及玻璃化冷冻技术4种不同保藏技术的研究进展进行了综述,在应用中总结优缺点,以期对后续相关工作的开展提供一定的科学依据。The amplification performance of biological detection reagents,especially the biological activity of enzyme reagents,plays a decisive role in the detection results of real-time quantitative PCR.It is crucial to adopt different preservation technolo⁃gies to maintain the biological activity of detection reagents,achieve long-term stable preservation and non-cold chain transporta⁃tion of biological detection reagents at room temperature,and ensure the stability,accuracy,specificity and sensitivity of detec⁃tion results.This paper reviewed the research progress of four different preservation technologies,including vacuum freeze-drying technology,spray drying technology,vitrification freezing technology,and summarized the advantages and disadvantages in ap⁃plication,in order to provide a certain scientific basis for the follow-up related work.

关 键 词：生物检测试剂 低温保藏技术 真空冷冻干燥技术 喷雾干燥技术 玻璃化冷冻技术 

分 类 号：Q-33[生物学]