西瓜蔗糖转运蛋白SUT家族的鉴定及其在果实发育和逆境响应中的表达分析  

Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

作　　者：寿伟松[1] 王铎 沈佳[1] 许昕阳 张跃建[1] 何艳军 SHOU Weisong;WANG Duo;SHEN Jia;XU Xinyang;ZHANG Yuejian;HE Yanjun(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2024年第1期94-102,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(32202478);浙江省自然科学基金(LY22C150010);浙江省农业新品种选育重大科技专项子课题任务(2021C02065-3-1-1)。

摘　　要：蔗糖转运蛋白(SUTs)作为蔗糖主动转运的主要载体,在分配同化物从源到库组织的转运中起着关键作用。尽管SUTs的特性及其生物学功能已在高等植物中得到了深入研究,但该基因家族尚未在西瓜中进行鉴定。本研究在西瓜基因组中共鉴定了4个ClSUT基因,并对其基因和蛋白结构、保守基序、亚细胞定位、染色体分布、进化关系、启动子元件等进行了全面分析。基因结构分析表明,亚族Ⅰ的ClSUT3和ClSUT4基因均只含有一个内含子。所有ClSUT均被预测定位在质膜中,它们均含有一个保守的MFS-2结构域,并且含有与之对应的5个保守基序。系统发育分析发现拟南芥、水稻和西瓜中的SUT分为5个亚族,西瓜ClSUT与拟南芥SUT的亲缘关系更近,被聚类在亚族Ⅰ、亚族Ⅱ和亚族Ⅴ。还在西瓜ClSUT基因的启动子中鉴定到一些与激素和逆境响应相关的作用元件。此外,转录组数据表明,西瓜ClSUT基因的表达对渗透和盐胁迫呈现多样性,很可能参与西瓜对这些逆境的响应。Sucrose transporter(SUTs) gene,as the main active transport vector of sucrose,plays a key role in the transport of allotment assimilates from source tissue to sink tissue.Although the characteristics and biological functions of SUTs have been well studied in higher plants,the SUT gene family has not been identified in watermelon.In this study,four ClSUT genes were identified in watermelon genome,and their gene and protein structure,conserved motifs,subcellular localization,chromosome distribution,evolutionary relationships and promoter cis-elements were comprehensively analyzed.Gene structure analysis showed that ClSUT3 and ClSUT4 grouped into subgroup Ⅰ and each only contained one intron.All ClSUT proteins were predicted to be localized in the plasma membrane.All ClSUT proteins contain a conserved MFS domain corresponding to five motifs.Phylogenetic analysis revealed that SUT proteins in Arabidopsis,rice and watermelon were divided into 5 subgroups.ClSUTs in watermelon have more closely relationship with SUT proteins in Arabidopsis and were clustered in subgroup Ⅰ,Ⅱ and Ⅴ.In addition,We also identified several functional cis-elements related to hormone and stress response in the watermelon ClSUT gene promoter.And transcriptome data indicated that watermelon ClSUT genes were variously expressed in response to osmotic and salt stress,which may be involved in watermelon responses to these stresses.

关 键 词：西瓜 蔗糖转运蛋白SUT 果实发育 非生物胁迫 表达分析 

分 类 号：S651[农业科学—果树学]