基于SRAP分子标记的华顶杜鹃遗传多样性  

Genetic diversity of Rhododendron huadingense based on SARP molecular marker

作　　者：吴倩 汤紫依 田盛野 何海叶 潘伟伟 王军峰 鲍洪华 张慧娟[1] 蒋明[1] WU Qian;TANG Ziyi;TIAN Shengye;HE Haiye;PAN Weiwei;WANG Junfeng;BAO Honghua;ZHANG Huijuan;JIANG Ming(College of Life Sciences,Taizhou University,Taizhou 318000,Zhejiang,China;Scientific Research Management Center,East China Medicinal Botanical Garden,Lishui 323000,Zhejiang,China;Luqiao Branch of Taizhou Municipal Ecology and Environment Bureau,Taizhou 318050,Zhejiang,China)

机构地区：[1]台州学院生命科学学院,浙江台州318000 [2]华东药用植物园科研管理中心,浙江丽水323000 [3]台州市生态环境保护局路桥分局,浙江台州318050

出　　处：《浙江农业学报》2024年第1期127-133,共7页Acta Agriculturae Zhejiangensis

基　　金：台州市大学生科技创新项目(2021年度);国家级大学生创新创业训练计划(202110350015)。

摘　　要：华顶杜鹃是浙江特有的珍稀濒危野生植物,分布区域十分狭窄,植株数量稀少,该植物亟待保护。以不同居群华顶杜鹃的叶片为材料,采用相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)分子标记技术进行遗传多样性研究。结果表明,从140个组合中筛选出8对扩增多态性高、条带清晰、重复性好的SRAP引物,利用这些引物对50份华顶杜鹃基因组DNA进行PCR扩增,共得到263个条带,其中多态性位点261个,多态性比率(PPB)为99.24%;在物种水平上,观测等位基因数(N_(a))为1.992 4,有效等位基因数(N_(e))为1.340 1,Nei’s基因多样性指数(H)为0.221 5,Shannon?s信息指数(I)为0.357 9,表明华顶杜鹃在物种水平上有较高的遗传多样性;在居群水平上,5个自然群体的遗传分化系数(G_(st))为0.459 6,基因流(N_(m))为0.587 8,表明居群间存在一定的基因分化和基因交流,而大部分遗传变异出现在居群内;UPGMA聚类结果表明,5个居群的样品可分为5组,与主成分分析结果一致。研究结果为后续开展该珍稀物种的保护和利用奠定了基础。Rhododendron huadingense is a rare and endangered wild plant species endemic to Zhejiang Province,China,with narrow distribution and very few individuals,thus this species needs to be protected.In this study,leaves of R.huadingense from different populations were collected,and SRAP(sequence related amplified polymorphism) molecular marker technology was applied to study its genetic diversity.Fifty genomic DNA samples were used for PCR amplification.The results indicated 8 pairs of primers with high levels of polymorphism,clear bands,and high reliability were screened from 140 primer pairs.Totally,263 bands were detected,including 261 polymorphic sites,and the percentage of the polymorphic band reached 99.24%.On species level,the number of observed alleles(N_a),number of effective alleles(N_e),Nei's gene diversity(H),and Shannon's information index(I) were 1.992 4,1.340 1,0.221 5,and 0.357 9,respectively,revealing its high genetic diversity on species level.On population level,the coefficient of genetic differentiation(G_(st)) and gene flow(N_m) were 0.459 6 and 0.587 8,respectively,indicating genetic differentiation and gene flow have occurred among populations,and most of the variation existed within populations.UPGMA results showed samples from 5 populations could be divided into 5 groups,which were consistent with principal component analysis results.Studies on genetic diversity of R.huadingense provided insights on protection and utilization of this rare plant species.

关 键 词：华顶杜鹃 遗传多样性 亲缘关系 

分 类 号：S685.21[农业科学—观赏园艺]