羊栖菜优良品系对高温的耐受性比较  

Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

作　　者：左晓洁 吴明江[1,2] 罗琳 马增岭[1,2] 庞观凤 陈斌斌 ZUO Xiaojie;WU Mingjiang;LUO Lin;MA Zengling;PANG Guanfeng;CHEN Binbin(College of Life and Environmental Science,Wenzhou University,Wenzhou 325035,Zhejiang,China;Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection,Wenzhou University,Wenzhou 325035,Zhejiang,China)

机构地区：[1]温州大学生命与环境科学学院,浙江温州325035 [2]温州大学浙江省水环境与海洋生物资源保护重点实验室,浙江温州325035

出　　处：《浙江农业学报》2024年第1期148-155,共8页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划(2018YFD0901500);国家自然科学基金(41706147,41876124)。

摘　　要：以3种羊栖菜优良品系和野生型羊栖菜为材料,在25℃培养,比较高温条件下羊栖菜的生长状况,为耐高温品系的筛选提供数据支持。结果表明,长时间高温培养可降低各品系羊栖菜的光合性能,抑制其硝态氮的吸收与同化相关酶的活性,降低可溶性蛋白和可溶性碳水化合物的含量,增强其暗呼吸速率,最终抑制藻体生长。在高温条件下,相比其他品系,梭形气囊型羊栖菜的最大电子传递速率和饱和光强放氧速率最高,过氧化物酶活性最高且显著增加,丙二醛含量并没有显著增加。总体上梭形气囊型羊栖菜受高温的影响较小,具有较强的耐受性。在实际养殖生产中可以推广梭形气囊型羊栖菜,研究结果可为后续的品种改良提供依据。Three excellent strains of Sargassum fusiforme and wild-type S.fusiforme were cultured at 25 ℃ to compare the growth status of S.fusiforme under high temperature conditions,and to provided data support for the screening of high temperature resistance strains.The results showed that long-term high temperature culture could reduce the photosynthetic performance of each strain of S.fusiforme,inhibit the activity of nitrate nitrogen absorption and assimilation related enzymes,reduce the content of soluble protein and soluble carbohydrate,enhance its dark respiration rate,and ultimately inhibit the growth of algae.Under high temperature condition,compared with other strains,the maximum electron transfer rate and saturated light intensity oxygen release rate of spindle vesicle type S.fusiforme were the highest,the peroxidase activity was the highest and increased significantly,while its malondialdehyde content did not increase significantly.In general,spindle vesicle type of S.fusiforme was less affected by high temperature and had strong tolerance.Spindle vesicle type of S.fusiforme could be popularized in actual breeding production,and the research results could provide a basis for subsequent variety improvement.

关 键 词：羊栖菜 种苗繁育 高温胁迫 光合作用 海藻养殖 

分 类 号：S968.42*5[农业科学—水产养殖]