安徽甜瓜和栝楼蔓枯病的病原菌鉴定及其有效药剂筛选  

Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides

作　　者：夏智杰 张雷 宋江华[2] 傅敏 张立新[1] XIA Zhijie;ZHANG Lei;SONG Jianghua;FU Min;ZHANG Lixin(Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,College of Plant Protection,Anhui Agricultural University,Hefei 230036,China;College of Horticulture,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学植物保护学院,植物病虫害生物学与绿色防控安徽普通高校重点实验室,安徽合肥230036 [2]安徽农业大学园艺学院,安徽合肥230036

出　　处：《浙江农业学报》2024年第1期168-176,共9页Acta Agriculturae Zhejiangensis

基　　金：安徽省重点研究与开发计划(202104b11020006)。

摘　　要：为明确安徽舒城和巢湖地区甜瓜和栝楼蔓枯病的病原菌种类,2021—2022年对该地区的甜瓜和栝楼蔓枯病的病组织进行病原菌的分离和培养,利用柯赫式法则验证其致病性。通过对分离菌株的形态学特征,以及ITS、LSU和TUB2多位点序列分析,确定引起甜瓜及栝楼蔓枯病的病原菌为亚隔孢壳菌Stagonosporopsis citrulli。采用菌丝生长速率法测定了7种杀菌剂对病原菌代表性菌株的室内毒力,结果显示,啶酰菌胺、咪鲜胺对其抑制效果较好,其EC_(50)值在0.010 6~0.043 8μg·mL^(-1),可优先选择这两种药剂进行轮换使用防治该病害。To identify the pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii,and screen effective fungicides for the management of this disease,symptomatic samples were collected from the fields located in Shucheng County and Chaohu City,Anhui Province in 2021 and 2022.Pure cultures were obtained consistently using tissue separation method,and five representative isolates were selected for morphological observation and molecular identification.Partial fragments of internal transcribed spacer(ITS) and large subunit(LSU) regions,beta-tublin 2(TUB2) genes of the isolates were amplified and sequenced.Pathogenicity assays were carried out to confirm Koch's postulates.The pathogen was finally identified as Stagonosporopsis citrulli based on morphological characteristics and multi-loci sequence analysis.The indoor toxicity test results indicated that boscalid and prochloraz exhibited relatively higher inhibitory effects among seven fungicides,with both EC_(50) ranging from 0.010 6 to 0.043 8 μg·mL~(-1).The two fungicides could be preferentially utilized in rotation to control the disease caused by S.citrulli.

关 键 词：甜瓜 栝楼 蔓枯病 杀菌剂 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]