盐胁迫对水培海巴戟幼苗生理特性的影响  

Effects of Salt Stress on Physiological Characteristics of Morinda ci-trifolia Seedlings in Hydroponics

作　　者：金璨 王璇[1] 宫树森 孙正海[1] 吴田[1] JIN Can;WANG Xuan;GONG Shusen;SUN Zhenghai;WU Tian(College of Landscape Architecture and Horticulture,Southwest Forestry University/Yunnan Functional Flower Resources and In-dustrialization Technology Engineering Research Center/State Forestry and Grassland Administration Southwest Landscape Archi-tecture Engineering Technology Research Center,Kunming,Yunnan 650224,China)

机构地区：[1]西南林业大学园林园艺学院/云南省功能性花卉资源及产业化技术工程研究中心/国家林业和草原局西南风景园林工程技术研究中心,云南昆明650224

出　　处：《热带作物学报》2024年第1期114-121,共8页Chinese Journal of Tropical Crops

基　　金：国家林业和草原局项目“诺丽果高产优质生产及加工技术推广示范”(No.[2019]27);云南省科技厅重点研发计划项目“特色优势植物木蝴蝶、诺丽、红掌集成培育技术老挝示范推广”(No.2019IB011)。

摘　　要：为明确海巴戟幼苗对盐的耐受范围及盐胁迫对海巴戟幼苗生长的影响,以水培180d的海巴戟苗为材料,基本培养基为1/8 MS液体培养基,其中分别加入质量分数为0、0.2%、0.4%、0.6%、0.8%和1.0%的NaCl进行胁迫处理。结果表明:随着NaCl胁迫浓度的增加与胁迫时间的推移,海巴戟叶片相对含水量(relative water content,RWC)与生物量逐渐降低,可溶性蛋白(solubleprotein,SP)、丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)活性均表现为先增加后减少的趋势。海巴戟幼苗的保护酶活性随着NaCl胁迫浓度的增加与胁迫时间的推移发生显著变化,在NaCl浓度0.8%以下范围,海巴戟可以通过调节渗透物质和抗氧化物酶来减轻伤害,保证生长;当NaCl浓度在0.8%及以上范围时,保护酶系统与细胞膜受损严重,进而影响海巴戟生长。因此,海巴戟幼苗可以在0.8%以下NaCl浓度范围的区域试种。In order to clarify the salt tolerance range of Morinda citrifolia seedlings and the effect of salt stress on the growth of M.citrifolia seedlings,the M.citrifolia seedlings cultured in water for 180 days were used as materials,and the basic medium was 1/8 MS liquid medium,in which NaCl with mass fractions of 0,0.2%,0.4%,0.6%,0.8%and 1.0%were added respectively for stress treatment.The results showed that with the increase of NaCl stress concentration and stress time,the relative water content(RWC)and biomass of M.citrifolia leaves gradually decreased,and the contents of soluble protein(SP),malondialdehyde(MDA)and the activities of superoxide dismutase(SOD),catalase(CAT),ascorbic acid peroxidase(APX)and peroxidase(POD)gradually increased first and then decreased.The protective enzyme activity of M.citrifolia seedlings changes significantly with the increase of NaCl stress concentration and the passage of stress time.In the range of NaCl concentration below 0.8%,M.citrifolia can reduce damage and ensure growth by regulating osmotic substances and antioxidant enzymes.When NaCl concentration is in the range of 0.8%or above,the protective enzyme system and cell membrane are seriously damaged,which affects the growth of M.citrifolia.Therefore,the seedlings of M.citrifolia can be tested in the area with NaCl concentration below 0.8%.

关 键 词：海巴戟 水培 盐胁迫 生理指标 保护酶活性 

分 类 号：S567.19[农业科学—中草药栽培]