木薯花叶病毒AC4蛋白与AtPARN互作研究  

The Interaction Between Cassava Mosaic Virus AC4 Protein and AtPARN

作　　者：刘琳玉 赵平娟[2] 符艳 刘志昕[2] 任艳利 张秀春[2] LIU Linyu;ZHAO Pingjuan;FU Yan;LIU Zhixin;REN Yanli;ZHANG Xiuchun(School of Biological and Geographical Sciences,Yili Normal University,Yining,Xinjiang 835000,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]伊犁师范大学生物与地理科学学院,新疆伊宁835000 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2024年第1期197-204,共8页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.322CXTD528,No.320RC709);中央级公益性科研院所基本科研业务费专项(No.1630052022021)。

摘　　要：木薯(Manihot esculenta Crantz)作为重要的热带作物,是全球六大粮食作物之一,并为全球近七亿人口提供主粮,然而病毒侵染引起的病害在全球范围内严重威胁木薯产业的发展。木薯花叶病毒病(cassava mosaic disease,CMD)是最有威胁的病害之一,造成严重的经济损失。斯里兰卡木薯花叶病毒(SriLankancassavamosaicvirus,SLCMV)是引发木薯花叶病的病原物之一,SLCMV是典型的双组分双生病毒,其基因组由DNA-A和DNA-B两个环状组分组成。无义介导的mRNA降解(nonsense-mediatedmRNAdecay,NMD)是真核细胞mRNA降解的主要途径之一,也是真核生物普遍存在的抗病毒防御机制。越来越多的研究显示,NMD不仅是真核生物重要的mRNA数量、质量调控机制,还与转录后基因沉默(post transcriptional gene silencing,PTGS)同样能降解病毒RNA,是真核生物普遍存在的抗病毒防御机制。NMD的mRNA衰减过程包括PTC的识别、脱腺苷酸、脱帽和最后的核酸外切酶降解4个过程,UPF1、PARN、DCP2和XRN4分别是上述4个过程中的关键蛋白。病毒是专性寄生生物,必须逃避或耐受寄主细胞的降解,才能成功感染。聚腺苷酸特异性核糖核酸酶[poly(A)-specific ribonuclease,PARN]是NMD信号通路的一个关键因子。目前,关于SLCMV抵御寄主NMD的分子机制尚不明确。本研究采用酵母双杂交(yeast two-hybrid system)和荧光双分子互补(bimolecular fluorescence complementation,BiFC)试验证明斯里兰卡木薯花叶病毒(SLCMV)编码的沉默抑制子AC4与拟南芥PARN相互作用。据此推测SLCMV AC4蛋白可能通过与AtPARN相互作用而抑制寄主NMD的病毒防御功能帮助病毒逃避或耐受寄主细胞的降解。研究结果为阐明木薯花叶病毒调控寄主NMD抗病毒防御功能的分子机理奠定基础。As an important tropical crop,Manihot esculenta Crantz is one of the top six global food crops and provides a staple food for nearly 700 million people worldwide.However,diseases caused by virus infection are a serious threat to the development of the cassava industry worldwide.Cassava mosaic disease(CMD)is one of the most threatening diseases,causing serious economic losses.Sri Lankan cassava mosaic virus(SLCMV)is one of the pathogens causing cassava mosaic disease.SLCMV is a typical two-component geminiviruses,and its genome consists of two circular components,DNA-A and DNA-B.Nonsensemediated mRNA decay(NMD)is one of the major pathways of mRNA degradation in eukaryotic cells and a preva-lent antiviral defense mechanism in eukaryotes.More and more studies have shown that NMD is not only an important mechanism for regulating mRNA quantity and quality in eukaryotes,but also degrades viral RNA as well as post transcriptional gene silencing(PTGS),which is a prevalent antiviral defense mechanism in eukaryotes.The mRNA decay process of NMD includes four processes:recognition of PTC,deadenylation,decapitation and final exonuclease degradation,and UPF1,PARN,DCP2 and XRN4 are the key proteins in these four processes,respectively.Viruses are specialized parasitic organisms that must escape or be resistant to degradation by host cells in order to successfully infect.Poly(A)-specific ribonuclease(PARN)is a key factor in the NMD signaling pathway.At present,the molecular mechanism of SLCMV resistance to host NMD is unclear.In this study,we will demonstrate that the silent suppressor AC4 encoded by SLCMV interacts with Arabidopsis PARN using yeast two-hybrid system and bimolecular fluorescence complementation(BiFC)experiments.It is hypothesized that the SLCMV AC4 protein may help the virus evade or tolerate degradation by the host cell by inhibiting the viral defense function of the host NMD through interaction with AtPARN.The results lay the foundation for elucidating the molecular mechanism by which cassava mosaic virus reg

关 键 词：斯里兰卡木薯花叶病毒(SLCMV) AC4蛋白 聚腺苷酸特异性核糖核酸酶(PARN) 酵母双杂交(Y2H) 荧光双分子互补(BiFC) 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]