不同因素对西藏阿旺绵羊胚胎冷冻与移植效果的影响  

Effects of Different Factors on Freezing andTransplantation of Awang Sheep Embryos

作　　者：虞莲 王幸栓 卓玛拉姆 邓增卓玛 杨志勇 王婵 李剑南 雷安民[1] YU Lian;WANG Xingshuan;Drolma Ran;Tenzin Drolma;YANG Zhiyong;WANG Chan;LI Jiannan;LEI Anmin(College of Veterinary Medicine,Northwest A&F University/Shaanxi Stem Cell Engineering and Technology Research Center,Yangling Shaanxi 712100,China;Tibet ChangduJinken Animal Husbandry Technology Co.,Ltd.,Changdu Xizang 854000,China)

机构地区：[1]西北农林科技大学动物医学院/陕西省干细胞工程技术研究中心,陕西杨凌712100 [2]西藏昌都津垦牧业科技有限责任公司,西藏昌都854000

出　　处：《西北农业学报》2024年第3期389-396,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省重点研发计划(2020NY-011);西藏昌都地区阿旺绵羊的胚胎冷冻研究与应用示范。

摘　　要：为探究西藏阿旺绵羊推行胚胎移植及胚胎冷冻技术的可行性,以阿旺绵羊为供体,澳湖羊为受体,探讨发情期内不同月份对阿旺绵羊超排效果的影响、不同发育阶段鲜胚和移植数量对母羊受胎率及产羔率的影响,以及冷冻方法对胚胎移植的影响。结果显示:发情期7月超排组平均可用胚数(3.95±4.23)显著低于10月超排组(5.45±4.03);阿旺绵羊胚胎移植2枚桑椹胚的受体妊娠率最高,达到81.81%,产羔率达57.58%,具有显著性差异;阿旺绵羊胚胎程序化冷冻组受体妊娠率为35.48%,显著优于玻璃化冷冻组受体妊娠率13.63%。以上结果表明,西藏推行阿旺绵羊胚胎移植技术的可行性较高。To explore the feasibility of embryo transfer and embryo freezing in Xizang Awang sheep.Awang sheep is used as donor and Aohu sheep as acceptor to study effect of different months of estrus on the superovulation in Awang sheep,the effect of fresh embryos at different developmental stages and the number of transferred embryos on the conception rate together with lambing rate of Awang sheep,as well as the effect of freezing methods on embryo transfer.The results showed that the number of embryos available in the superovulation group in July in estrus(3.95±4.23)is evidently lower than that in the superovulation group in October(5.45±4.03).The pregnancy rate of two morulae transferred from Awang sheep is the highest at 81.81%,and the lambing rate is 57.58%.The pregnancy rate of Awang sheep embryos in programmed freezing group is 35.48%,while the vitrification group is 13.63%.Therefore,Awang sheep embryo transfer technology is feasible in Xizang.

关 键 词：阿旺绵羊 发育阶段 玻璃化冷冻 程序化冷冻 

分 类 号：S826.3[农业科学—畜牧学]