甘肃玉米灰斑病病原鉴定及遗传多样性分析  

Identification and Genetic Diversity Analysis ofPathogen of Maize Gray Leaf Spot in Gansu

作　　者：戴子淙 高建昊 张小杰 洪流[2] 王春明[2] 周天旺[2] 陈杰新 郭成 DAI Zicong;GAO Jianhao;ZHANG Xiaojie;HONG Liu;WANG Chunming;ZHOU Tianwang;CHEN Jiexin;GUO Cheng(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070 China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;College of Grassland Science,Gansu Agricultural University,Lanzhou 730070;Pingliang Academy of Agricultural Sciences,Pingliang Gansu 744000 China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070 [2]甘肃省农业科学院植物保护研究所,兰州730070 [3]甘肃农业大学草业学院,兰州730070 [4]平凉市农业科学院,甘肃平凉744000

出　　处：《西北农业学报》2024年第3期562-572,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：嘉峪关市科技计划(22-26);甘肃省青年科技托举工程人才(2020-18)。

摘　　要：为掌握甘肃地区玉米灰斑病的发生分布、病原种类及遗传多样性,于2021-2022年调查了该病害在甘肃的分布范围,对采集的灰斑病样品进行分离与鉴定,并采用ISSR分子标记技术对甘肃玉米灰斑病菌的遗传多样性进行分析。结果表明,玉米灰斑病在甘肃陇南和陇东大部分地区均有发生,并已扩展至陇中地区;经形态学和分子生物学鉴定甘肃地区玉米灰斑病病原菌为玉米尾孢(Cercospora zeina);16条ISSR引物共扩增出74条条带,多态性条带73条,比例为98.65%。甘肃省陇南地区种群遗传多样性最为丰富,且与陕西省的遗传距离最小(0.021 4)、遗传相似度最高(0.978 9),陇东地区次之。在遗传相似系数为0.63时,可以将所有菌株分为4个亚群,亚群1中有28个菌株,其中包括16株陇南地区菌株、5株陇东地区菌株和7株来自陕西地区的菌株;亚群2中只有1个陇南地区菌株;亚群3中有10个菌株,包括陇南地区4个、陇东地区1个和5个陕西菌株;亚群4有2个菌株,陇东地区和陕西菌株各1个,4大类群分别包含来自不同地域的菌株。以上结果说明,同陇东地区相比,陇南地区与陕西地区的菌株遗传相似度更高,且亚群内遗传多样性与地理来源之间无明显的相关性。In order to understand the occurrence and distribution of maize gray leaf spot and its pathogen species and genetic diversity in Gansu Province,the distribution range of the disease in Gansu Province was investigated during 2021 to 2022.The samples of gray leaf spot were isolated and identified,and the genetic diversity of the pathogen was analyzed by ISSR molecular marker technology.The results showed that the gray leaf spot occurred in most areas of eastern and southern Gansu,and extended to central Gansu.The pathogen of gray leaf spot was identified as Cercospora zeina by morphology and molecular biology.A total of 74 bands were amplified by 16 ISSR primers,with 73 polymorphic bands,accounting for 98.65%.The genetic diversity of Longnan population was the most abundant,and the minimum genetic distance from Shaanxi Province was 0.0214,and the highest genetic similarity was 0.9789,followed by Longdong region.When the genetic similarity coefficient was 0.63,all strains could be divided into four subgroups.There were 28 strains in subgroup 1,including 16 strains from Longnan,five strains from East Gansu and seven strains from Shaanxi.There was only one strain from Longnan in subgroup 2.There were 10 strains in subgroup 3,including four strains from Longnan,one strain from East Gansu and five strain from Shaanxi.There were two strains in subgroup 4,one strain from Shaanxi,and one strain from Longdong.The four groups contained strains from different regions.The above results showed that compared with East Gansu area,the genetic similarity of strains from Longnan and Shaanxi was higher,and there was no significant correlation between genetic diversity and geographical origin.

关 键 词：甘肃 玉米灰斑病 分布 玉米尾孢 遗传多样性 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]