基于氨基化石墨烯-金@铂纳米粒子复合材料的无酶型电化学免疫传感器的构建及应用  

Construction and Application of Enzyme-Free Electrochemical Immunosensor Based on Amino-Fossil Graphene-Au@Pt Nanoparticle Composites

作　　者：赵丽芳 于晓菲 滕龙 齐云 刘鹏飞 侯斌 张娟 Zhao Lifang;Yu Xiaofei;Teng Long;Qi Yun;Liu Pengfei;Hou Bin;Zhang Juan(Shandong Institute for Product Quality Inspection,Jinan 250100,China;Shandong Huankeyuan Environmental Engineering Co.,Ltd.,Jinan 250109,China;Binzhou Inspection and Testing Center,Binzhou 256600,China)

机构地区：[1]山东省产品质量检验研究院,山东济南250100 [2]山东省环科院环境工程有限公司,山东济南250109 [3]滨州市检验检测中心,山东滨州256600

出　　处：《山东化工》2024年第1期20-22,29,共4页Shandong Chemical Industry

基　　金：食品用洗涤剂中防腐剂的检测技术体系构建(项目编号:2021ZJKY023)。

摘　　要：报道了一种新型的甲胎蛋白检测用无酶型电化学免疫传感器。这种免疫传感器利用金@铂纳米粒子对过氧化氢的高催化性能和石墨烯的高导电性能,增加了电子转移能力和信号放大能力,摆脱了免疫检测中对酶的需要,从而使无酶检测成为可能。这种新型免疫传感器对甲胎蛋白的检测限为0.00003 pg/mL,线性范围为在0.0001~10 pg/mL。A novel electrochemical immunosensor capable of enzyme-free detection of alpha fetoprotein(AFP)is reported.This immunosensor uses the high catalytic properties of catalyzed gold-platinum nanoparticles against hydrogen peroxide and the high conductive performance of graphene to increase electron transfer capacity and signal amplification,escaping the need for enzymes in immunodetection,thus enabling enzyme-free detection.With a low detection limit at 0.00003 pg/mL,this novel immunosensor worked well over the broad linear range of 0.0001~10 pg/mL.

关 键 词：甲胎蛋白 无酶型 电化学免疫传感器 

分 类 号：O657.1[理学—分析化学]