PUVA治疗诱导皮肤线粒体DNA 4977 bp缺失突变累积的研究  

Study on accumulations of mitochondrial 4977bp deletion of skin with PUVA treatment

作　　者：刘仲荣[1] 刘荣卿[2] 张国威[1] 阎国富[1] 何云志[1] 

机构地区：[1]第三军医大学附属二院皮肤科 [2]第三军医大学附属一院皮肤科,重庆400038

出　　处：《临床皮肤科杂志》2002年第12期743-745,共3页Journal of Clinical Dermatology

摘　　要：为探讨线粒体DNA(mitochondrialDNA,mtDNA)4977bp缺失突变与皮肤光老化之间的关系,采用三条引物PCR的方法检测长波紫外线光化学疗法(PUVA)治疗的银屑病患者背部非皮损区皮肤mtDNA4977bp缺失突变的累积。结果发现,在PUVA治疗期间3～21天、治疗后1～6个月和治疗后6个月以上的各组活检标本中,发生4977bp缺失突变的mtDNA占总mtDNA比例分别为0.06,0.12和0.19,和未经PUVA治疗的对照组比较均有显著性差异(P<0.01)。表明线粒体DNA4977bp缺失突变累积与皮肤光老化密切相关,可能作为衡量皮肤紫外线损伤程度的分子生物学标志。To investigate the relationships between mitochondrial4977bp deletion and skin photoaging.The approach of three-primers polymerase chain reaction(PCR)was used to detect accumulations of mitochondrial4977bp deletion of the non-lesion back skin of vulgar psoriatic patients with PUVA treatment.The results manifested,of the species of biopsies with PUVA treat-ment 3～21days,after PUVA treatment 1-6months and after PUVA treatment above6months,the proportions of mtDNA car-ried mito chondrial4977bp deletion of the total mtDNA were0.06,0.12and0.19,respectively.Compared with the control group,the differences were significant (P<0.01).The resutts showed that accumulations of mitochondrial4977bp deletion was closely asso ciated with skin photoaging and may be a biomolecular marker for evaluation of the level of ultraviolet damage of skin.

关 键 词：PUVA 治疗 线粒体DNA 4977BP缺失 皮肤光老化 分子生物学 银屑病 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]