滋膵降糖方介导Fetuin B-AMPK/ACC通路对高脂诱导肥胖小鼠肝脏胰岛素抵抗的调控机制研究  

Study on Regulatory Mechanism of Fetuin B-AMPK/ACC Pathway Mediated by Zicui Jiangtang Prescription on Insulin Resistance in Liver of High-fat-induced Obese Mice

作　　者：刘曼曼 冯珍凤 胡春平[1] 陈见纺[1] 沈怡华 高俊凤 严军[1] LIU Manman;FENG Zhenfeng;HU Chunping;CHEN Jianfang;SHEN Yihua;GAO Junfeng;YAN Jun(Shanghai City Jiading District Hospital of Traditional Chinese Medicine,Shanghai 201899,China;Graduate School,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海市嘉定区中医医院,上海201899 [2]上海中医药大学研究生院,上海201203

出　　处：《西部中医药》2024年第2期14-19,共6页Western Journal of Traditional Chinese Medicine

基　　金：上海市嘉定区科委项目(JDKW-2019-W17);上海市“十四五”中医特色专科项目(ZYTSZK1-10);上海市嘉定区卫健委项目(2023-KY-ZYY-03)。

摘　　要：目的:探讨滋膵降糖方通过Fetuin B-AMPK/ACC通路改善肥胖C57BL/6J小鼠胰岛素抵抗的机制。方法:高脂饮食喂养C57BL/6J小鼠,构建胰岛素抵抗模型,随机分为模型组、滋膵降糖方组、二甲双胍组、联合组(滋膵降糖方+二甲双胍),正常饮食小鼠为空白对照组。观察各组小鼠体质量、肝脏质量、葡萄糖耐量、胰岛素抵抗指数,肝脏Fetuin B、AMPK、ACC mRNA及肝脏Fetuin B、AMPK_(α1)、ACC、P-AMPK_(αT183/T172)、P-ACC_(S79)蛋白表达。结果:滋膵降糖方可改善小鼠葡萄糖耐量,减小曲线下面积,降低胰岛素抵抗指数,下调肝脏质量和肝体比,降低肝脏Fetuin B、ACC mRNA和Fetuin B蛋白表达,上调AMPK mRNA和P-AMPK_(αT183/T172)/AMPK_(α1)、P-ACCs79/ACC蛋白水平。结论:滋膵降糖方可能通过Fetuin B-AMPK/ACC信号通路减轻肥胖小鼠肝脏胰岛素抵抗。Objective:To explore the mechanism of improving insulin resistance in obese C57BL/6J mice via Fetuin B-AMPK/ACC pathway medicated by Zicui Jiangtang prescription.Methods:The models with insulin resistance(IR)were established by feeding C57BL/6J mice high-fat diet,and they were randomly allocated to the model group,Zicui Jiangtang prescription group,metformin group,the combination group(Zicui Jiangtang prescription+metformin),the mice fed normal diet were chosen as blank control group.To observe the mice's body mass,liver mass,glucose tolerance,insulin resistance index(IRI),the expressions of liver Fetuin B,AMPK,ACC mRNA,liver Fetuin B,AMPK_(α1),ACC,P-AMPK_(αT183/T172) and P-ACC_(S79) protein.Results:Zicui Jiangtang prescription could improve the mice′glucose tolerance,reduce area under the curve,lower IRI,liver mass and hepatocrit,cut down the expressions of liver Fetuin B,ACC mRNA and Fetuin B protein,up regulate the levels of AMPK mRNA,P-AMPK_(αT183/T172) and P-ACC_(S79)/ACC protein.Conclusion:Zicui Jiangtang prescription could alleviate IR of liver in obese mice possibly via Fetuin B-AMPK/ACC signaling pathway.

关 键 词：滋膵降糖方 Fetuin B AMPK/ACC 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]