益气养阴方对动脉粥样硬化大鼠免疫炎症因子和胸主动脉内、中膜厚度的影响  

Effects of Benefiting-Qi Nourishing-Yin Prescription on Immunoinflammatory Factor,Thoracic Aortic Intima-media Thickness in Atherosclerosis Rats

作　　者：谢心[1] 徐浣白[2] 樊月月 罗浩 吴坚[1] XIE Xin;XU Huanbai;FAN Yueyue;LUO Hao;WU Jian(Shanghai TCM-integrated Hospital,Shanghai University of TCM,Shanghai 200082,China;Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]上海市中西医结合医院,上海200082 [2]上海市第一人民医院,上海200080

出　　处：《西部中医药》2024年第2期27-31,共5页Western Journal of Traditional Chinese Medicine

基　　金：上海市科学技术委员会医学引导类科研项目(14401931700);上海市虹口区卫健委科研项目(虹卫1702-02);上海市海派中医流派传承人才培养项目(RCPY004)。

摘　　要：目的:观察益气养阴方对动脉粥样硬化大鼠治疗前后免疫炎症因子的表达水平及对胸主动脉内、中膜厚度变化的影响。方法:46只4周龄SPF级Wistar雄性大鼠,随机选出10只为空白组,只给予基础饲料,其余36只采用主动脉内膜球囊拉伤术加高脂饲料喂养完成动脉粥样硬化大鼠模型。造模成功后的30只随机分为模型组、益气养阴方组(中药组)和瑞舒伐他汀组(西药组),每组10只。空白组予以基础饲料喂养,中药组给予益气养阴方,西药组给予瑞舒伐他汀钙片。给药6周后胸主动脉剪取粥样斑块留作标本,检测超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、转化生长因子β_(1)(transforming growth factor β_(1),TGF-β_(1))、白细胞介素8(interleukin-8,IL-8)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)的表达水平及胸主动脉内、中膜厚度及病理变化。结果:用SNK法两两比较,与模型组比较,中药组、西药组在hs-CRP、TGF-β_(1)、IL-8、MMP-9的表达水平方面均明显降低(P<0.05),胸主动脉内、中膜厚度的变化差异有统计学差异(P<0.05)。益气养阴方组大鼠胸、腹主动脉各层细胞结构的组织修复方面优于模型组,病变明显减轻。结论:益气养阴方对动脉粥样硬化大鼠治疗前后hs-CRP、TGF-β_(1)、IL-8、MMP-9的表达水平及颈动脉内中膜厚度方面均有明显改善作用,为临床对动脉粥样硬化的干预治疗提供一个研究方向。Objective:To observe the influence of benefiting-Qi nourishing-Yin prescription on the expressions of immunoinflammatory factor,thoracic aortic intima-media thickness in atherosclerosis rats before and after the treatment.Methods:Ten rats were randomly selected as the blank group among 46 four-week SPF grade Wistar male rats,and they accepted basic feed,the remaining 36 ones were prepared into atherosclerosis rats by aortic endothelial balloon straining and high-fat feed.30 rats after successfully modeling were randomized into the model group,benefiting-Qi nourishing-Yin prescription group(Chinese medicine group)and rosuvastatin group(Western medicine group),ten rats in each group.The blank group was fed with basic feeds,Chinese medicine group took benefiting-Qi nourishing-Yin prescription and Western medicine group rosuvastatin calcium tablets.After six weeks of administration,thoracic aorta was cut to retain atherosclerotic plaque as the samples,to detect the levels of hs-CRP,TGF-β_(1),IL-8 and MMP-9,thoracic aortic intima-media thickness and pathological changes.Results:SNK method was used to compare two groups,compared with the model group,the levels of hs-CRP,TGF-β_(1),IL-8 and MMP-9 were lowered obviously in Chinese medicine group and Western medicine group(P<0.05),the difference had statistical meaning in thoracic aortic intima-media thickness(P<0.05).Chinese medicine group was better than the model group in the aspects of repair of cellular structures in various layers of rat thoracic and abdominal aorta,and the pathological changes were relieved obviously.Conclusion:Benefiting-Qi nourishing-Yin herbs could markedly improve the expressions of hs-CRP,TGF-β_(1),IL-8 and MMP-9,thoracic aortic intima-media thickness before and after the treatment in atherosclerosis rats,and it could offer a research direction for clinical intervention of atherosclerosis.

关 键 词：动脉粥样硬化 益气养阴方 炎症因子 胸主动脉内膜 胸主动脉中膜 大鼠 动物实验 

分 类 号：R541.4[医药卫生—心血管疾病]