麻黄附子细辛汤治疗缓慢性心律失常的网络药理学机制  

Mechanism of Mahuang Fuzi Xixin Decoction in Treating Bradyarrhythmia Based on Network Pharmacology

作　　者：马春丽 于艺 迟倩慧 张蕾[1] 刘颖[1] MA Chunli;YU Yi;CHI Qianhui;ZHANG Lei;LIU Ying(College of Acupuncture and Tuina,Shandong University of Traditional Chinese Medicine,Jinan 250300,China)

机构地区：[1]山东中医药大学针灸推拿学院,山东济南250300

出　　处：《西部中医药》2024年第2期104-110,共7页Western Journal of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划项目(2017-023)。

摘　　要：目的:基于网络药理学探讨麻黄附子细辛汤治疗缓慢性心律失常的作用机制。方法:通过TCMSP数据库结合OB、DL、HL检索麻黄附子细辛汤中麻黄、附子、细辛的化学成分和作用靶点,并通过Uniprot数据库将靶点校正为基因名,运用GeneCards数据库获取缓慢性心律失常的相关靶点与基因。通过Venny图将缓慢性心率失常的靶点和药物靶点进行分析,筛选出交集靶点,并借助Cytoscape 3.6.1软件构建化合物靶点相互作用图;根据STRING数据库构建蛋白质相互作用的PPI网络图;利用David数据库对交集靶点进行GO富集分析和KEGG通路富集分析。结果:筛选出麻黄附子细辛汤治疗缓慢性心律失常的36个有效活性成分,关键化合物为槲皮苷和山柰酚;与治疗缓慢性心律失常相关的靶点65个,主要靶点有IL-6、TNF、VEGFA、CXCL8、IL-1B等,主要涉及腺苷酸环化酶激活肾上腺素能受体信号通路、一氧化氮生物合成过程的正向调节、去甲肾上腺素-肾上腺素血管收缩参与全身动脉血压的调节、血管生成的积极调节等生物工程,通过参与TNF、Toll样受体、钙、cGMP-PKG等信号通路治疗缓慢性心律失常。结论:该研究从多角度探索了麻黄附子细辛汤治疗缓慢性心律失常的可能作用机制,认为麻黄附子细辛汤通过多成分、多靶点、多途径治疗缓慢性心律失常,为临床上治疗该病提供了有力的依据。Objective:To explore the mechanism of Mahuang Fuzi Xixin decoction in treating bradyarrhythmia based on network pharmacology.Methods:Chemical ingredients and the targets of Mahuang(ephedrae herba),Fuzi(aconiti lateralis radix praeparata)and Xixin(Asari radix et rhizoma)in Mahuang Fuzi Xixin decoction were searched from TCMSP,OB,DL and HL,the targets were corrected to gene names via the Uniprot database,the related targets and genes of bradyarrhythmia were obtained using GeneCards database.The intersection targets were screened after analyzing the targets of the disease and drug targets via Venny diagram,compound-target interaction diagram was built via Cytoscape 3.6.1 software;PPI network was constructed based on STRING database;GO enrichment analysis and KEGG pathway enrichment analysis of the intersection targets were carried out utilizing David database.Results:All 36 effective active ingredients of Mahuang Fuzi Xixin Decoction in treating the disease have been screened,and the key compounds are quercetin and kaempferol;there were 65 targets related to the treatment of the disease,and the main ones contained IL-6,TNF,VEGFA,CXCL8 and IL-1B,mainly involving the adrenergic receptor signaling pathway activated by adenylate cyclase,positive regulation of nitric oxide biosynthetic processes,norepinephrine-adrenergic vasoconstriction involved in the regulation of systemic arterial blood pressure,positive regulation of angiogenesis,which could treat the disease through participating in TNF signaling pathway,Toll-like receptor signaling pathway,calcium signaling pathway and cGMP-PKG signaling pathway.Conclusion:The study has been a multifaceted investigation of the potential mechanism of Mahuang Fuzi Xixin decoction in the treatment of bradyarrhythmia,it is believed that the decoction could treat the disease from multi-ingredient,multi-target and multi-way,and it could produce the powerful evidence for clinical treatment of the disease.

关 键 词：心律失常 缓慢性 麻黄附子细辛汤 网络药理学 

分 类 号：R541[医药卫生—心血管疾病]