麻射安金丸对慢性阻塞性肺疾病气道黏液高分泌大鼠模型气道炎症及黏蛋白5AC和γ-氨基丁酸A受体α亚基表达水平的影响  

Effects of Mashe Anjin Pills on airway inflammation and expression levels of mucin 5AC andγ-aminobutyric acid A receptorαsubunit in rat model with hypersecretion of airway mucus in chronic obstructive pulmonary disease

作　　者：尚艳花 邓敬华 张晓阳 马真 王玉栋 SHANG Yanhua;DENG Jinghua;ZHANG Xiaoyang;MA Zhen;WANG Yudong(Department of Pulmonology,Hengshui Hospital of Traditional Chinese Medicine,Hebei Province,Hengshui 053000,China)

机构地区：[1]河北省衡水市中医医院肺病科,河北衡水053000

出　　处：《中国医药导报》2023年第36期10-15,共6页China Medical Herald

基　　金：河北省中医药管理局中医药类科研计划项目(2022274)。

摘　　要：目的 探讨麻射安金丸对慢性阻塞性肺疾病(COPD)气道黏液高分泌大鼠模型气道炎症及黏蛋白5AC(MUC5AC)和γ-氨基丁酸A受体α亚基(GABAARα)表达水平的影响。方法 将102只体重(195±20)g的7周龄雄性SD大鼠按随机数字表法分为正常组、模型组、氨溴索阳性对照组及中药低、中、高剂量组,每组17只。除正常组外,其余各组均采用脂多糖滴入联合香烟烟雾暴露方式建立COPD气道黏液高分泌大鼠模型。造模成功后,氨溴索阳性对照组给予氨溴索溶液[54 mg/(kg·d)],中药低、中、高剂量组分别给予4.375、8.750、17.500 g/(kg·d)麻射安金丸(汤剂),正常组和模型组以等容量生理盐水灌胃,六组均连续灌胃28 d。实验结束后采用苏木精-伊红染色、过碘酸希夫染色观察各组肺组织病理学变化,酶联免疫吸附试验检测各组血清炎症因子水平和肺泡灌洗液细胞因子水平,采用蛋白质印迹法检测各组肺组织中MUC5AC、GABAARα含量。结果 模型组气道及肺组织病理变化较重,白细胞介素-1β(IL-1β)、中性粒细胞弹性蛋白酶(NE)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)、组织金属蛋白酶抑制物-1(TIMP-1)、基质金属蛋白酶-9(MMP-9)、MUC5AC、GABAARα水平均高于正常组,差异有统计学意义(P<0.05)。中药低、中、高剂量组气道及肺组织病理变化轻于模型组,IL-1β、NE、TNF-α、TGF-β1、TIMP-1、MMP-9、MUC5AC、GABAARα水平均低于模型组,差异有统计学意义(P<0.05)。中药中剂量组IL-1β、NE、MMP-9、GABAARα均低于中药低剂量组;中药高剂量组IL-1β、NE、TNF-α、TGF-β1、TIMP-1、MMP-9、MUC5AC、GABAARα表达均低于中药低、中剂量组,差异有统计学意义(P<0.05)。结论 麻射安金丸能改善COPD气道黏液高分泌状态,抑制气道重塑,可能与其减轻炎症损伤,抑制MUC5AC、GABAARα表达有关。Objective To investigate the effects of Mashe Anjin Pills on airway inflammation and the expression levels of mucin 5AC(MUC5AC)andγ-aminobutyric acid A receptorαsubunit(GABAARα)in rat model with hypersecretion of airway mucus in chronic obstructive pulmonary disease(COPD).Methods A total of 1027-week-old male SD rats with body weight of(195±20)g were divided into normal group,model group,Ambroxol positive control group,and Chinese medicine low-dose,medium-dose,high-dose groups by random number table method,with 17 rats in each group.Except for the normal group,the other groups were treated with lipopolysaccharide drops combined with cigarette smoke exposure to establish COPD airway mucus hypersecretion rat model.After successful modeling,Ambroxol positive control group was given Ambroxol solution(54 mg/[kg·d]),Chinese medicine low-dose,medium-dose,high-dose groups were given 4.375,8.750,and 17.500 g/(kg·d)Mashe Anjin Pills(decoction),respectively,normal group and model group were given equal volume of normal saline,and all six groups were given continuous gavage for 28 days.After the experiment,hematoxylin-eosin staining and periodic acid Schiff stain were used to observe the pathological changes of lung tissue in each group.Serum inflammatory factors and alveolar lavage fluid cytokine levels were detected by enzyme-linked immunosorbent assay.The contents of MUC5AC and GABAARαin lung tissue were detected by Western blot.Results The pathological changes of airway and lung tissues in the model group were more serious.The levels of interleukin-1β(IL-1β),neutrophil elastase(NE),tumor necrosis factor-α(TNF-α),transforming growth factor-β1(TGF-β1),tissue metalloproteinase-1(TIMP-1),matrix metalloproteinase-9(MMP-9),MUC5AC,GABAARαof model group were higher than those of normal group,the differences were statistically significant(P<0.05).The pathological changes of airway and lung tissues in Chinese medicine low-dose,medium-dose,high-dose groups were less than those in model group,and the levels of IL-1β,N

关 键 词：麻射安金丸 慢性阻塞性肺疾病 黏蛋白5AC γ-氨基丁酸A受体α亚基 

分 类 号：R285.5[医药卫生—中药学]