检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:郭丹[1] 张月芬[1] 戴雅彬 黄慧[1] 熊蔚 GUO Dan;ZHANG Yuefen;DAI Yabin;HUANG Hui;XIONG Wei(Fujian Health College,Fuzhou Fujian 350101,China;Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang Jiangxi 330029,China)
机构地区:[1]福建卫生职业技术学院,福建福州350101 [2]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029
出 处:《药品评价》2023年第10期1193-1196,共4页Drug Evaluation
基 金:福建省卫健委青年科研课题(2019-2-26)。
摘 要:目的评价藤茶总黄酮对大气细颗粒物(PM2.5)所致大鼠肺损伤的作用。方法雄性Wistar大鼠35只随机分为5组:对照组,模型组,藤茶总黄酮(TF)高、中、低剂量组,每组7只。模型组、TF各组气管滴注PM2.5混悬液10 mg/kg,每周1次,共4次,对照组则以生理盐水进行气管滴注。滴注次日起,对照组、模型组以0.5%羧甲基纤维素钠灌胃,TF组以不同剂量(500、250、125 mg/kg)藤茶总黄酮灌胃,每周5次,共4周。收集大鼠肺组织、肺泡灌洗液(BALF),观察肺组织病理形态,检测肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px),酶联免疫吸附试验(ELISA)检测BALF中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)。结果与对照组相比,模型组大鼠肺组织损伤明显,SOD、GSH-Px活性显著降低,MDA含量、BALF中TNF-α、IL-6、IL-1β水平显著升高。在中高剂量TF干预下,PM2.5肺损伤模型大鼠的肺组织损伤减轻,SOD、GSH-Px活性显著增强,MDA含量、BALF中TNF-α、IL-6、IL-1β水平显著下降,差异有统计学意义。结论藤茶总黄酮对PM2.5所致大鼠肺损伤具有一定的保护作用,这一作用与减轻大鼠肺部的氧化应激和炎症反应有关。Objective To evaluate the effect of tengcha flavonoids on lung injury of rats induced by atmospheric fine particulate matter(PM2.5).Methods 35 male Wistar rats were randomly divided into five groups:the control group,model group,tengcha flavonoids(TF)high-dose,medium-dose,and low-dose group,with 7 rats in each group.The model and TF groups were exposed to PM2.5 suspension by intratracheal instillation at a dose of 10 mg/kg once a week for four weeks,while the control group was instilled with normal saline.On the next day of modeling,the control and model groups were administered 0.5%sodium carboxymethyl cellulose solution,whereas the TF groups were given TF suspension in dosages of 500,250,and 125 mg/kg five times a week for four weeks.The lung tissues and bronchoalveolar lavage fluid(BALF)of rats were collected.The histopathological changes of lungs were observed.The levels of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in lung tissue were detected.The contents of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in BALF were measured by enzyme-linked immunosorbent assay(ELISA).Results Compared to the control group,the model group showed obvious injury in lung tissues.The activities of SOD and GSH-Px in lung tissues were significantly decreased,the content of MDA in lung tissues and the levels of TNF-α,IL-6 and IL-1βin BALF were significantly increased in the model group.Under the treatment of TF with medium and high dose,the degree of lung injury was alleviated,the activities of SOD and GSH-Px were increased,the contents of MDA in lung tissues and the levels of TNF-α,IL-6 and IL-1βin BALF were decreased significantly.Conclusion Tengcha flavonoids has a certain protective effect on lung injury of rats induced by PM2.5,which is associated with reduction of oxidative stress and inflammatory response.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.15