荧光定量PCR法与涂片抗酸染色法在活动性肺结核早期诊断中的应用价值  

Application Value of Fluorescence Quantitative PCR Method and Smear Acid-Fast Staining Method in Early Detection of Active Pulmonary Tuberculosis

作　　者：刘炜钊 冼英杰 李秀群 Liu Weizhao;Xian Yingjie;Li Xiuqun(Qingyuan Qingxin District People's Hospital,Qingyuan 511800,Guangdong Province,China)

机构地区：[1]清远市清新区人民医院,广东清远511800

出　　处：《中国社区医师》2023年第34期70-72,共3页Chinese Community Doctors

摘　　要：目的:探讨荧光定量聚合酶链式反应(PCR)法与涂片抗酸染色法在活动性肺结核早期诊断中的应用价值。方法:选取2020年1月—2023年7月清远市清新区人民医院收治的69例疑似活动性结核患者作为研究对象,收集痰液标本并实施荧光定量PCR与涂片抗酸染色检测,以临床综合诊断结果为“金标准”,比较两种检验方式的诊断效能。结果:临床综合诊断活动性肺结核35例,阳性率为50.72%(35/69)。荧光定量PCR法诊断活动性肺结核阳性率高于涂片抗酸染色法,差异有统计学意义(P<0.001)。荧光定量PCR法诊断早期活动性肺结核的特异度、准确度、灵敏度高于涂片抗酸染色法,差异有统计学意义(P<0.05)。结论:与涂片抗酸染色法相比,荧光定量PCR法诊断早期活动性肺结核的阳性率、特异度、准确度、灵敏度更高,值得临床应用并予以推广。Objective:To explore the application value of fluorescence quantitative polymerase chain reaction(PCR)method and smear acid-fast staining method in the early detection of active pulmonary tuberculosis.Methods:A total of 69 patients with suspected active tuberculosis who were treated in Qingyuan Qingxin District People's Hospital from January 2020 to July 2023 were selected as the study subjects.Sputum specimens were collected.Fluorescent quantitative PCR and smear acid-fast staining were performed.Clinical comprehensive diagnosis results were taken as the golden standard to compare the diagnostic efficiency of the two methods.Results:A total of 35 patients were diagnosed as active pulmonary tuberculosis by clinical comprehensive manifestation,with a positive rate of 50.72%(35/69).The positive rate of fluorescence quantitative PCR in the diagnosis of active pulmonary tuberculosis was higher than that of smear acid-fast staining,and the difference was statistically significant(P<0.001).The specificity,accuracy and sensitivity of fluorescence quantitative PCR in the diagnosis of early active pulmonary tuberculosis were higher than those of smear acid-fast staining,and the difference was statistically significant(P<0.05).Conclusion:Compared with smear acid-fast staining,fluorescent quantitative PCR method in the early diagnosis of active pulmonary tuberculosis has a higher positive rate,specificity,accuracy,and sensitivity,and it is worthy of clinical application and promotion.

关 键 词：活动性肺结核 荧光定量PCR 涂片抗酸染色 

分 类 号：R521[医药卫生—内科学]