HPLC指纹图谱结合一测多评的土贝母标准汤剂质量研究  

Study on the quality of Bolbostemmatis Rhizoma standard decoction by HPLC fingerprint combined with quantitative analysis of multi-components by single marker

作　　者：李振雨 周湘媛 马懿飞 丘娴 何民友 陈向东 孙冬梅 Li Zhenyu;Zhou Xiangyuan;Ma Yifei;Qiu Xian;He Minyou;Chen Xiangdong;Sun Dongmei(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《国际中医中药杂志》2024年第1期76-83,共8页International Journal of Traditional Chinese Medicine

基　　金：国家工业和信息化部2019年产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：目的采用HPLC法建立土贝母标准汤剂指纹图谱,并采用一测多评法对结构相似的3个有效成分含量进行测定,评价土贝母标准汤剂的质量。方法采用HPLC法建立15批土贝母标准汤剂指纹图谱,色谱柱为Waters XBridge苯基柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液,梯度洗脱。以共有峰的相对峰面积为依据,进行聚类分析和主成分分析,并采用与指纹图谱相同的方法建立土贝母标准汤剂土贝母苷甲、乙、丙的一测多评法。结果土贝母标准汤剂指纹图谱有14个共有峰,其中峰3为L-色氨酸,峰11为土贝母苷乙,峰12为土贝母苷丙,峰13为土贝母苷甲;聚类分析和主成分分析将15批不同产地的土贝母标准汤剂分为3类;一测多评法通过系统适用性考察,与外标法测定结果无显著差异。结论该方法准确、可靠,专属性良好,可有效评价土贝母标准汤剂的质量。Objective To establish the HPLC fingerprint of Bolbostemmatis Rhizoma standard decoction;To determine the three effective components with similar structure by quantitative analysis of multi-components by single marker(QAMS);To evaluate the quality of Bolbostemmatis Rhizoma standard decoction.MethodssHPLC was adopted to establish the fingerprints of 15 batches of Bolbostemmatis Rhizoma standard decoction.The Chromatographic column was Waters XBridge Phenyl(4.6 mmx250 mm,5μm).The mobile phaseewas acetonitrile-0.1%phosphoric acid solution with gradient elution.Cluster analysis(HCA)and principal component analysis(PCA)were conducted based on the relative peak area of common peaks.The same method as the fingerprint was used to establish QAMS of tubeimoside A,B,C on Bolbostemmatis Rhizoma standard decoction.Results There were 14 common peaks in the fingerprint of Bolbostemmatis Rhizoma standard decoction.It was confirmed that the peak 3 was L-tryptophan,the peak 11 was tubeimoside B,the peak 12 was tubeimoside C,and the peak 13 was tubeimoside A.15 batches of Bolbostemmatis Rhizoma standard decoction from different origins were divided into 3 categories by HCA and PCA.There was no significant difference between QAMS andthe external standard method(ESM)through the systemnsuitabilityinspection.Conclusion This method is accurate,reliable and has good specificity,which can effectively evaluate the quality of Bolbostemmatis Rhizoma standard decoction.

关 键 词：土贝母 标准汤剂 指纹图谱 土贝母苷甲 土贝母苷乙 土贝母苷丙 一测多评 

分 类 号：R284.1[医药卫生—中药学]