银甲丸加减方对衣原体持续性感染输卵管炎模型小鼠输卵管病理及炎性因子的影响  

Effects of Modified Yinjia Pills on Pathological and Inflammatory Factors of Fallopian Tube in Mice with Chlamydia Persistent Infection salpingitis model…

作　　者：罗梅[1,2,3] 宋志华 田雪莲[4] 张园 周思韵 胡荣静[1] 邹凌凤 Luo Mei;Song Zhihua;TianXuelian;Zhang Yuan;Zhou Siyun;Hu Rongjing;Zou Lingfeng

机构地区：[1]重庆市中医院妇科,重庆400021 [2]重庆中医药学院,重庆402760 [3]成都中医药大学第四临床医学院,四川成都610021 [4]成都中医药大学临床医学院,四川成都610075

出　　处：《实用中医药杂志》2024年第1期5-8,共4页Journal of Practical Traditional Chinese Medicine

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX1030);2021年重庆市中青年医学高端人才项目(渝卫人(2022)15号文件);重庆市中医名科建设项目。

摘　　要：目的:观察银甲丸加减方对生殖道衣原体(CT)持续性感染输卵管炎小鼠输卵管病理、CT清除率及炎性因子IL-10、IFN-γ和TGF-β的调节作用。方法:36只BALB/c小鼠随机分为6组,除空白组以外,其余组小鼠阴道内均接种衣原体建立CT持续性感染模型。造模成功后分组给药,第28天处死,HE染色观察小鼠上生殖道组织病理改变,q RT-PCR测定小鼠生殖道CT含量,ELISA检测小鼠血清IL-10、IFN-γ和TGF-β表达水平。结果:与空白对照组比较,模型组CT基因表达量显著增高(P<0.01)。与模型组比较银甲丸加减方中、高剂量组和阳性药物(阿奇霉素)对照组CT基因表达显著降低(P<0.05)。IL-10、IFN-γ含量显著上调(P<0.05),TGF-β含量显著下降(P<0.05)。结论:银甲丸加减方有助于缓解衣原体生殖道感染模型小鼠的输卵管炎病理状态,促进持续性感染CT的清除,其机制可能是通过调节细胞因子IL-10、IFN-γ和TGF-β的表达来实现。Objective:To observe the effect of modified Yinjia Pills on fallopian tube pathology,CT clearance rate and inflammatory factors IL-10,IFN-γand TGF-βin mice with genital chlamydia(CT)persistent infection salpingitis.Methods:Thirty-six BALB/c mice were randomly divided into 6 groups.Except for the blank group,the mice in the other groups were inoculated with Chlamydia in the vagina to establish a CT persistent infection model.After successful modeling,the mice were divided into groups and sacrificed on the 28 th day.HE staining was used to observe the pathological changes of the upper genital tract of mice.The content of CT in the genital tract of mice was determined by qRT-PCR.The expression levels of IL-10,IFN-γand TGF-βin serum of mice were detected by ELISA.Results:Compared with the blank control group,the expression of CT gene in the model group was significantly increased(P<0.01).Compared with the model group,the expression of CT gene in the middle and high dose groups of Yinjia Pills and the positive drug(azithromycin)control group was significantly decreased(P<0.05).The contents of IL-10 and IFN-γwere significantly up-regulated(P<0.05),and the content of TGF-βwas significantly decreased(P<0.05).Conclusion:Modified Yinjia Pills could help alleviate the pathological state of salpingitis in mice with chlamydia infection of genital tract and promote the clearance of CT in persistent infection.The mechanism may be achieved by regulating the expression of cytokines IL-10,IFN-γand TGF-β.

关 键 词：银甲丸加减方 小鼠 输卵管炎 生殖道衣原体持续性感染 IL-10 IFN-Γ TGF-β 

分 类 号：R271.917.5[医药卫生—中西医结合]