基于响应面分析的高抗原活性猪链球菌2型疫苗培养基的研制与效果评价研究  

Development and effect evaluation of Streptococcus suis type 2 vaccine medium with high antigenic activity based on response surface analysis

作　　者：苗森 牛得权 张阳阳 刘翔 蒋富凤 张菊梅 万强 吴清平 MIAO Sen;NIU De-quan;ZHANG Yang-yang;LIU Xiang;JIANG Fu-feng;ZHANG Ju-mei;WAN Qiang;WU Qing-ping

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601 [2]广东省科学院微生物研究所华南应用微生物国家重点实验室广东省微生物安全与健康重点实验室农业农村部农业微生物组学与精准应用重点实验室,广东广州510070 [3]广东环凯生物科技有限公司,广东肇庆526238

出　　处：《饲料研究》2024年第1期86-92,共7页Feed Research

基　　金：国家重点研发项目(项目编号:2021YFA0910200);广东省重点研发计划项目(项目编号:2022B1111040002);广东省科学院创新发展专项(项目编号:2020GDASYL-20200103025)。

摘　　要：试验旨在研制具有高抗原活性的猪链球菌2型(Streptococcus suis type 2,SS2)疫苗培养基。研究以SS2 CVCC60615株为试验对象,通过单因素试验、Plackett-Burman试验、最陡爬坡试验和响应面分析得到SS2疫苗培养基。培养基发酵验证结果显示,SS2疫苗培养基菌液最大活菌数为1.09×10^(9) CFU/mL,是TSB培养基最大活菌数的2.41倍。测定SS2不同时间点的抗原活性发现,在SS2发酵培养过程中当活菌数达到最大值后的1~3 h内细菌的抗原活性最高。小鼠感染试验表明,SS2抗原活性最高时制备的灭活疫苗与商品化灭活疫苗相比具有更高的免疫球蛋白G(IgG)抗体效价和疫苗保护率。研究表明,采用研制得到的高抗原活性SS2疫苗培养基制备的灭活疫苗具有IgG抗体效价高、免疫保护力强的优点。The study aimed to develop a Streptococcus suis type 2(SS2)vaccine medium with a high antigenic activity.SS2 CVCC60615 strain was used as the experimental object.The SS2 vaccine medium was developed by a single factor test,Plackett-Burman test,steepest ascent test and response surface analysis.The culture medium fermentation verification results showed that the maximum viable count in the culture medium of SS2 vaccine was 1.09×10^(9) CFU/mL,which was 2.41 times higher than that obtained by using TSB medium.Through the determination of SS2 antigenic activity at different time points,it was found that the antigenic activity of bacteria was the highest within 1~3 h after the count of viable bacteria reached the maximum in the fermentation culture process of SS2.The mice infection test showed that the inactivated vaccine prepared when the SS2 antigen activity was the highest had a higher IgG antibody titer and a vaccine protection rate than the commercial inactivated vaccine.The study indicates that the inactivated vaccine prepared using the developed high antigen activity SS2 vaccine culture medium has the advantages of high IgG antibody titer and strong immune protection force.

关 键 词：猪链球菌2型 响应面分析 高抗原活性 疫苗培养基 

分 类 号：S851[农业科学—预防兽医学]