Distance-basedα-amylase biosensor fabricated with amylopectin-coated mesoporous membrane  

作　　者：Binglu Zhao Mashooq Khan Yulin Liu Wenjun Tai Chongyang Mu Wenli Wu Mei Zhao Yaohong Ma Li Yu Jin-Ming Lin Qiongzheng Hu 

机构地区：[1]Qilu University of Technology(Shandong Academy of Sciences),Shandong Analysis and Test Center,Ji’nan 250014,China [2]School of Pharmaceutical Sciences,Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250014,China [3]Key Laboratory of Colloid and Interface Chemistry,Shandong University,Ministry of Education,Ji’nan 250100,China [4]Key Laboratory for Biosensors of Shandong Province,Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250353,China [5]Beijing Key Laboratory of Microanalytical Methods and Instrumentation,MOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China

出　　处：《Chinese Chemical Letters》2024年第1期446-450,共5页中国化学快报（英文版）

基　　金：the National Key R&D Program of China(Nos.2021YFB3201200,2021YFB3201202);the Taishan Scholar Program(No.tsqn201812088);he Natural Science Foundation of Shandong Province(No.ZR2022YQ12);the Shandong Scientific and Technical Small and Medium-sized Enterprises Innovation Capacity Improvement Project(No.2022TSGC2533);the Science,Education and Industry Integration Innovation Pilot Project from Qilu University of Technology(Shandong Academy of Sciences)(No.2022JBZ02-04).

摘　　要：Paper-based biosensors are widely employed in point-of-care testing(POCT)due to their convenience,portability,low cost,and ease of use.This study reports an integrated distance-based paper biosensor fabricated with a mesoporous membrane coated with stimuli-responsive polymer.The detection ofα-amylase(AMY)using amylopectin-coated mesoporous membrane is demonstrated as an example.After introducing the AMY solution,it is observed that the aqueous solution flows along the paper strip due to AMY-catalyzed hydrolysis of amylopectin.The flow distance is proportional to the concentration of AMY with a detection limit as low as 4 mU/mL.In addition,the detection of AMY is demonstrated in human serum.Furthermore,the inhibitory effect of acarbose on AMY is evaluated.This reagent-free and disposable biosensor allows single-step rapid detection of the analyte.This approach is very promising for the development of user-friendly,equipment-free,and cost-effective biosensors with remarkable sensitivity and excellent selectivity for disease diagnosis and hypoglycemic drug screening.

关 键 词：Paper biosensor Α-AMYLASE Stimuli-responsive polymer Mesoporous membrane DISTANCE Point-of-care testing 

分 类 号：R318[医药卫生—生物医学工程] TQ317[医药卫生—基础医学] TP212.3[化学工程—高聚物工业]