骨髓肿瘤坏死因子-α水平在成人急性B淋巴细胞白血病预后评估中的作用研究  

Preliminary Study of Bone Marrow Tumor Necrosis Factor⁃alpha(TNF⁃α)Level and Its Prognostic Value in Adult B⁃cell Acute Lymphoblastic Leukemia

作　　者：文志浩 金依颖 杨莹[1] 张欣[1] 白杨娟[1] 廖红艳[1] WEN Zhi-hao;JIN Yi-ying;YANG Ying;ZHANG Xin;BAI Yang-juan;LIAO Hong-yan(Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院实验医学科,成都610041

出　　处：《中国生物工程杂志》2023年第12期86-93,共8页China Biotechnology

基　　金：四川省科技厅项目(2023NSFSC1484);四川大学华西医院学科卓越发展1·3·5工程临床研究基金(2023HXFH034)资助项目。

摘　　要：目的:探究成人急性B淋巴细胞白血病(B-ALL)患者骨髓肿瘤坏死因子-α(TNF-α)水平及其在患者化疗效果及肿瘤复发评估中的作用。方法:前瞻性地收集了107名B-ALL患者(疾病组)、25名健康供者(健康对照组)和77名其他类型血液肿瘤患者(疾病对照组)的外周血和骨髓血浆样本。通过化学发光免疫分析法测定血浆TNF-α浓度;流式细胞术测定缓解和复发患者白血病细胞半乳糖凝集素-9(Gal-9)表达情况;TNF-α与患者原代细胞体外共培养,检测Gal-9表达情况。结果:B-ALL患者骨髓TNF-α水平升高且TNF-α水平与首次化疗缓解情况有关,首次化疗完全缓解者初诊时的骨髓TNF-α浓度较非完全缓解者更低;复发患者白血病细胞Gal-9表达显著上调;TNF-α在体外可诱导Gal-9表达上调。结论:揭示了成人B-ALL患者骨髓及外周血TNF-α水平升高,并诱导白血病细胞Gal-9表达上调促进疾病进展和复发。Objective:This study intends to investigate the levels of bone marrow tumor necrosis factor⁃alpha(TNF⁃α)in adult acute B⁃lymphoblastic leukemia(B⁃ALL)patients and the role of TNF⁃αin the efficacy of chemotherapy and tumor recurrence.Methods:Peripheral blood and bone marrow plasma samples were prospectively collected from 107 patients with B⁃ALL(disease group),25 healthy donors(healthy controls),and 77 patients with other types of hematologic neoplasms(disease controls).Plasma TNF⁃αconcentration was determined by chemiluminescence immunoassay.Gal⁃9 expression in leukemia cells from remission and relapse patients was determined by flow cytometry and TNF⁃αwas co⁃cultured in vitro with primary cells from the patients to detect the changes in the expression of Gal⁃9.Results:Bone marrow TNF⁃αlevels were elevated in B⁃ALL patients and TNF⁃αlevels correlate with whether or not they are in remission from the first chemotherapy treatment.Bone marrow TNF⁃αconcentrations at the time of initial diagnosis were lower in the first⁃time chemotherapy complete remission patients compared to those in non⁃complete remission patients.The leukemia cell Gal⁃9 was significantly up⁃regulated in relapsed patients and TNF⁃αinduced Gal⁃9 up⁃regulation in vitro.Conclusions:This study reveals that bone marrow and peripheral blood TNF⁃αlevels are elevated in adult B⁃ALL patients and induce up⁃regulation of Gal⁃9 in leukemia cells to promote disease progression and tumor relapse.

关 键 词：急性B淋巴细胞白血病 肿瘤坏死因子 半乳糖凝集素-9 

分 类 号：Q939.91[生物学—微生物学]