AtWUS过表达诱导分蘖洋葱体细胞胚高频发生  

AtWUS Promotes High Frequency Somatic Embryogenesis Potato Onion(Allium cepa L.var.Aggregatum G.Don)

作　　者：徐启江 黄俊慧 何涛[2] 邢志勇 杨安妮[1] 黄云彤 XU Qijiang;HUANG Junhui;HE Tao;XING Zhiyong;YANG Anni;HUANG Yuntong(Modern Industrial College of Biomedicine and Great Health,Youjiang Medical University for Nationalities,Baise,Guangxi 533000;School of Laboratory Medicine,Youjiang Medical University for Nationalities,Baise,Guangxi 533000;Baise Institute of Scientific and Technical Information,Baise,Guangxi 533000)

机构地区：[1]右江民族医学院生物医药与大健康现代产业学院,广西百色533000 [2]右江民族医学院医学检验学院,广西百色533000 [3]百色市科学技术信息研究所,广西百色533000

出　　处：《北方园艺》2024年第1期24-30,共7页Northern Horticulture

基　　金：广西自然科学基金面上资助项目(2023GXNSFAA026289);2022年百色市科学研究与技术开发计划资助项目(百科2022002);2023年第二批百色市科学研究与技术开发计划自筹经费资助项目(百科20232021)。

摘　　要：以分蘖洋葱茎尖外植体诱导的愈伤组织为试材,采用农杆菌介导法,将外源基因AtWUS导入分蘖洋葱愈伤组织中,研究AtWUS对分蘖洋葱愈伤组织诱导体细胞胚发生的影响,以期建立分蘖洋葱体细胞胚高频发生体系。结果表明:过表达AtWUS可以显著促进分蘖洋葱愈伤组织的体细胞胚发生率(大约6倍),91%的双极性体细胞胚具有发育形成完整植株能力。RT-PCR分析结果表明,AtWUS在体细胞胚和转基因植株中高丰度表达。Taking callus induced from shoot apex explants of potato onion(Allium cepa var.aggregatum Don)as test material,the foreign gene AtWUS genetic transformation was carried out by the agrobacterium-mediated transformation method with potato onion callus.The effects of AtWUS on somatic embryogenesis(SE)of potato onion calluswas studied,in order to develop a high-frequency SE regeneration systemvia over-expressing AtWUS from in potato onion callus.The results showed that overexpression of the AtWUS gene incallus promoted,as the percentage of explants giving rise to embryogenic tissues was significantly higher(×6)when AtWUS gene was over-expressed than in the control.Bipolar somatic embryos resulted 91%complete plant regeneration.RT-PCR analysis showed that AtWUS gene was highly expressed in somatic embryos and transgenic plants.

关 键 词：分蘖洋葱 茎尖外植体 愈伤组织 体细胞胚发生 AtWUS基因 

分 类 号：S633.2[农业科学—蔬菜学]