北京地区不同品种草莓种苗中草莓轻型黄边病毒的检测与分析  

Detection and Analysis of Strawberry Mild Yellow Edge Virus in Different Varieties of Strawberry Seedlings in Beijing Area

作　　者：张家兴 王秋实 周宇 黄雅琪 严滕 尚巧霞[1] ZHANG Jiaxing;WANG Qiushi;ZHOU Yu;HUANG Yaqi;YAN Teng;SHANG Qiaoxia(College of Bioscience and Resource Environment,Beijing University of Agriculture/Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs,Beijing 102206;Agricultural Technology Extension Station of Changping District in Beijing,Beijing 102200)

机构地区：[1]北京农学院生物与资源环境学院,农业农村部华北都市农业重点实验室,北京102206 [2]北京市昌平区农业技术推广站,北京102200

出　　处：《北方园艺》2024年第1期38-44,共7页Northern Horticulture

基　　金：北京市教委科技重点资助项目(KZ202210020028);北京农学院青年教师科研创新能力提升计划资助项目(QJKC2022002)。

摘　　要：以产自北京地区的36个品种的草莓种苗为试材,采用田间调查和RT-PCR方法,研究草莓轻型黄边病毒(SMYEV)在不同品种草莓种苗上的影响,以期为草莓品种选育和推广工作提供参考依据。结果表明:采集的431株草莓种苗中检出SMYEV阳性样品30份,病毒检出率为6.96%。其中,8个品种的草莓种苗中检出SMYEV,检出率为6.5%~23.1%,而其他28个品种均未检出。通过基因克隆、序列测定和比对分析,获得3个北京分离物的SMYEV外壳蛋白(CP)核苷酸序列,北京分离物BJHY253与美国草莓分离物WSU1988CP基因序列的同源性为98.63%;北京分离物BJHYZ83与阿根廷草莓分离物Berra-2CP基因序列的同源性为99.04%,北京分离物BJLYN与中国福建草莓分离物FJ2 CP基因序列的同源性为98.63%。通过对不同草莓品种中的SMYEV进行检测分析和序列测定,发现SMYEV在不同品种草莓种苗中的检出率存在差异,北京地区草莓种苗中SMYEV序列具有多样性,属于不同的株系,这些结果可以为草莓的田间生产和病毒防控提供数据支持。The effects of strawberry mild yellow edge virus(SMYEV)on different varieties of strawberry seedlings were studied by field investigation and RT-PCR using 36 varieties of strawberry seedlings from Beijing area as materials,in order to provide reference for the breeding and popularization of strawberry varieties.The results showed that among the 431 strawberry seedlings collected,30 SMYEV positive samples were detected,and the virus detection rate was 6.96%.SMYEV was detected from 8 varieties of strawberry seedlings,and the detection rate was 6.5%-23.1%,while no SMYEV were found in the other 28 varieties.The nucleotide sequences of SMYEV coat protein(CP)of three Beijing isolates were obtained by gene cloning,sequencing and alignment analysis.The homology of CP gene sequence between Beijing isolate BJHY253 and American isolate WSU1988 was 98.63%.The homology of CP gene sequence between Beijing isolate BJHYZ83 and Argentina isolate Berra-2 was 99.04%.The homology of CP gene sequence between Beijing isolate BJLYN and Fujian isolate FJ2 was 98.63%.In this study,SMYEV in different strawberry varieties was detected and sequenced.It was found that the detection rate of SMYEV in various strawberry varieties was different.The SMYEV sequences in strawberry seedlings in Beijing were diverse and belonged to different strains.These results will provide data support for field production and virus prevention and control of strawberry.

关 键 词：草莓轻型黄边病毒 不同品种 RT-PCR 病毒检测 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]