自然杀伤T细胞样细胞在系统性硬化病患者中的表达及意义  

Significance of natural killer T like cells in peripheral blood of patients with systemic sclerosis

作　　者：李燕[1,2] 马向波 周新华 王昱澍 贾园 赵静[1] 相晓红[1] 李云[1] 孙峰[1] 李英妮 Li Yan;Ma Xiangbo;Zhou Xinhua;Wang Yushu;Jia Yuan;Zhao Jing;Xiang Xiaohong;Li Yun;Sun Feng;Li Yingni(Department of Rheumatology and Immunology,Peking University People's Hospital,Beijing 100044,China;Jinzhong First People's Hospital Clinical Laboratory,Jinzhong 030600,China;Department of Rheumatology and Immunology,the First Hospital of Handan City,Handn 056002,China;Clinical Laboratory,the Third Hospital of Nanchang City,Nanchang 330009,China;Inspection Center,Qingdao University Medical College Affiliated Yuhuangding Hospital,Yantai 264000,China)

机构地区：[1]北京大学人民医院风湿免疫科,北京100044 [2]晋中市第一人民医院检验科,晋中030600 [3]邯郸市第一医院风湿免疫科,邯郸056002 [4]南昌市第三医院检验科,南昌330009 [5]青岛大学医学院附属毓璜顶医院检验中心,烟台264000

出　　处：《中华风湿病学杂志》2023年第11期728-732,I0003,共6页Chinese Journal of Rheumatology

基　　金：国家自然科学基金(81871281);北京大学人民医院研究与发展基金(RDC2019-01)。

摘　　要：目的研究外周血自然杀伤T细胞样(NKT-like)细胞在SSc患者中的表达,探索NKT-like细胞与SSc实验室指标和临床指标之间的相关性,进而探讨NKT-like细胞在SSc发生发展中的作用。方法选取2018年12月至2019年12月在北京大学人民医院风湿免疫科门诊和住院就诊的46例SSc患者(SSc组),年龄及性别与之匹配的30名健康体检者,作为健康对照组(HC组)。采用流式细胞术收集SSc组和HC组外周血NKT-like细胞和其他淋巴细胞亚群的细胞数量和百分比。同时采用多种实验方法测定其他实验室指标。相关性分析采用Pearson或Spearman分析,2组间差异采用Mann-WhitneyU检验,或采用Fisher确切概率法,对NKT-like细胞和其他临床及实验室指标进行相关统计学分析。结果与HC组[165(72,226)个/μl]相比,SSc组外周血中NKT-like细胞数量[30(19,58)个/μl]及比例明显降低,差异具有统计学意义(Z=-5.69,P<0.001)。相关性分析可见,NKT-like细胞数量与总T细胞(r=0.56,P<0.001)、CD4+T细胞(r=0.42,P=0.004)、CD8+T细胞(r=0.60,P<0.001)、B细胞(r=0.50,P<0.001)及NK细胞(r=0.33,P=0.024)呈正相关;NKT-like细胞所占淋巴细胞百分比与CD8+T细胞所占百分比呈正相关(r=0.34,P=0.020),与其他淋巴细胞百分比无相关性(P>0.05)。NKT-like细胞降低组ESR较NKT-like细胞正常组显著升高[15(9,28)mm/1 h与8(4,16)mm/1 h,Z=-2.04,P=0.042]。NKT-like细胞数量与ESR呈负相关(r=-0.34,P=0.019)。结论SSc患者外周血NKT-like细胞存在数量和比例明显降低,NKT-like细胞不仅与多种淋巴细胞亚群呈正相关,而且与反映疾病活动度的ESR呈负相关。NKT-like细胞可作为监测SSc疾病活动的一种指标。Objective To study the expression of peripheral blood NKT-like cells in patients with systemic sclerosis(SSc),to explore the correlation between NKT-like cells and laboratory and clinical indicators of systemic sclerosis,and investigate the role of NKT-like cells in the occurrence and development of Systemic sclerosis.Methods Forty-six SSc patients(SSc group)were enrolled from Department of Rheumatology and Immunology of Peking University People's Hospital during December 2018 to December 2019.Thirty healthy subjects with matched age and sex were selected as healthy control group(HC group).The cell count and percentage of NKT-like cells and other lymphocyte subsets in peripheral blood were detected by flow cytometry.At the same time,other laboratory indexes were determined by different methods.Spearman's correlation analysis,Pearson's correlation analysis,Man-Whitney U test and Fisher's exact test were used to analyze the difference and correlation between NKT-like cells and other clinical and laboratory indicators.Results Compared with HC group[165(72,226)cells/μl],the cell count of NKT-like cells in peripheral blood of SSc group[30(19,58)cells/μl]was significantly decreased(Z=-5.69,P<0.001).Correlation analysis showed that the cell count of NKT-like cells was positively correlated with total T lymphocytes(r=0.56,P<0.001),CD4+T cells(r=0.42,P=0.004),CD8+T cells(r=0.60,P<0.001),B cells(r=0.50,P<0.001)and NK cells(r=0.33,P=0.024),respectively.The percentage of NKT-like cells in lymphocytes was also positively correlated with the percentage of CD8+T cells(r=0.34,P=0.020),but not significantly correlated with other subset of lymphocytes.The ESR of the NKT-like cell decreased group was significantly higher than that of the NKT-like normal group[15(9,28)mm/1 h vs 8(4,16)mm/1 h,Z=-2.04,P=0.042].Moreover,the cell count of NKT-like cells was negatively correlated with ESR(r=-0.34,P=0.019).Conclusion The cell count and percentage of NKT-like cells in peripheral blood of SSc patients decreased significantly.NKT-like cell

关 键 词：硬皮病 系统性 淋巴细胞 血沉 自然杀伤T细胞 

分 类 号：R593.2[医药卫生—内科学]