微藻不同光合荧光参数对氯化消毒副产物毒性响应特性  

Response Characteristics of Different Photosynthetic Fluorescence Parameters of Microalgae to Toxicity of Chlorinated Disinfection By-products

作　　者：甘婷婷 殷高方 赵南京[1,2,3] 汪颖[1,2,3] 谭小璇 叶紫琪 Gan Tingting;Yin Gaofang;Zhao Nanjing;Wang Ying;Tan Xiaoxuan;Ye Ziqi(Key Laboratory of Environmental Optics and Technology,Chinese Academy of Sciences,Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,Anhui,China;University of Science and Technology of China,Hefei 230026,Anhui,China;Key Laboratory of Optical Monitoring Technology for Environment of Anhui Province,Hefei 230031,Anhui,China;Hefei University of Technology,Hefei 230009,Anhui,China)

机构地区：[1]中国科学院合肥物质科学研究院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室,安徽合肥230031 [2]中国科学技术大学,安徽合肥230026 [3]安徽省环境光学监测技术重点实验室,安徽合肥230031 [4]合肥工业大学,安徽合肥230009

出　　处：《光学学报》2023年第24期386-395,共10页Acta Optica Sinica

基　　金：国家重点研发计划(2021YFC3200100,2022YFC3103901);安徽省科技重大专项(202003a07020007,202203a07020002)。

摘　　要：以淡水微藻中的蛋白核小球藻为受试生物,以典型毒性氯化消毒副产物氯乙酸及三氯乙腈为研究对象,采用荧光动力学方法研究了4种光合荧光参数F_(v)/F_(m)、F_(v)/F_(o)、F_(m)/F_(o)(F_(o)为初始荧光强度,F_(m)为最大荧光强度,F_(v)=F_(m)-F_(o))及PI_(ABS)对两种消毒副产物的响应规律,并分别从对低质量浓度与等质量浓度消毒副产物响应性能、10%效应质量浓度(EC_(10))与50%效应质量浓度(EC_(50))值4个方面对比了4个光合荧光参数的响应灵敏性。结果表明:4个参数对两种消毒副产物都具有质量浓度响应特性,抑制率与消毒副产物质量浓度之间都具有较好的Logistic曲线型剂量-效应关系,修正相关系数R^(2)_(adj)均大于0.993,其中PI_(ABS)的R^(2)_(adj)最大;4个参数对两种消毒副产物毒性的响应灵敏性从大到小的排序均为PI_(ABS)、F_(v)/F_(o)、F_(m)/F_(o)、F_(v)/F_(m),因此PI_(ABS)是基于荧光动力学方法检测水体氯化消毒副产物毒性较为灵敏的响应指标。该研究结果为发展水体消毒副产物毒性的现场快速检测方法与技术提供了重要参考。Objective Due to the advantages such as good inactivation effect on pathogenic microorganisms and low usage cost,chlorinated disinfectants have been widely employed for disinfection in various aspects.However,the large amount of chlorinated disinfection by-products produced by chlorinated disinfectants have biological toxicity,and they will have toxic effects on aquatic organisms and pose potential threats to the aquatic ecosystem once entering aquatic environment.Therefore,the development of on-site and rapid detection methods for the toxicity of chlorinated disinfection by-products in water is of significance for ensuring the aquatic environment safety.As the main primary producers and energy converters in aquatic ecosystems,microalgae play an important role in maintaining the balance and stability of aquatic ecosystems and indicating the water environment quality.Additionally,the microalgae photosynthesis has a rapid response characteristic to the toxicity of pollutants in water.Based on this,fluorescence kinetics technique which could rapidly and non-destructively detect the photosynthetic status of living plants has good development prospects in rapid toxicity detection of chlorinated disinfection by-products in water.However,at present,it is still unclear which photosynthetic fluorescence parameter obtained based on fluorescence kinetics technique can serve as the optimal response indicator for rapidly and sensitively detecting the toxicity of chlorinated disinfection by-products.Therefore,we study the response characteristics of different photosynthetic fluorescence parameters to the toxicity of chlorinated disinfection by-products for determining the optimal response indicator with sensitive response characteristics to chlorinated disinfection by-products.This is of practical significance for the development of on-site and rapid detection methods for the toxicity of chlorinated disinfection by-products based on fluorescence kinetics technology.Methods We employ a common freshwater microalgae Chlorella pyr

关 键 词：光谱学 荧光 氯化消毒副产物 生物毒性 微藻 荧光动力学 光合荧光参数 

分 类 号：X832[环境科学与工程—环境工程]